| Project/Area Number |
16390506
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKATO Tsuyoshi The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (90171454)
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| Co-Investigator(Kenkyū-buntansha) |
CHIKAZU Daichi The University of Tokyo, Faculty of Medicine, Assistant, 医学部附属病院, 助手 (30343122)
HOSHI Kazuto The University of Tokyo, Faculty of Medicine, Visiting Assistant Professor, 医学部附属病院, 寄附講座教員 (30344451)
KOYAMA Hiroyuki The University of Tokyo, Faculty of Medicine, Visiting Assistant Professor, 医学部附属病院, 寄附講座教員 (10241994)
NISHIYAMA Nobuhiro The University of Tokyo, Faculty of Medicine, Assistant, 大学院・医学系研究科, 助手 (10372385)
KATAOKA Kazunori The University of Tokyo, Faculty of Medicine, Professor, 大学院・医学系研究科, 教授 (00130245)
江口 智明 東京大学, 医学部附属病院, 講師 (00302688)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2005: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2004: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Polymer nano-micellsb / Reverse transfection / collagen gel / Bone regeneration / Gene delivery / Vascular tissue / RGD / RGDペプチド / Artery wall binding peptide / Reverse transfect |
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| Research Abstract |
(1)Development of non-viral vector system for specific gene delivery
To develop nano-micelle gene vector for specific gene delivery, we added ligand peptides (RGD peptide, ring-formed RGD peptide or artery wall binding peptide) to the poly-ion complex nano-micelle system (RGD micelle, ring-RGD micelle and AWBP micelle). The evaluations using several culture cell lines showed that gene transfer efficiency by ring-RGD micelle was significantly higher than those by micelle without ligand and RGD micelle. Further, in culture smooth muscle cells, AWBP micelle showed remarkable gene transfer as compared with other micelles.
(2)Developmento of gene delivery method for vascularization of reconstructive tissue
Although we assessed in vivo gene delivery efficiency to muscle tissue by using RGD micelle and ring-RGD micelle, there was no significant advantage in RGD micelle and ring-RGD micelle as compared with non-ligand micelle. We also tested gene delivery effect by AWBP micelle to the injured arterial wall, but no significant effect was detected. Therefore, we established new gene transfer approach of nano-micelle gene vector by using reverse transfection method. Further, we also carried out the evaluation of new poly-ion micelle, PEG-DET, and observed favorable gene delivery to rabbit carotid artery.
(3)Vascularization of regenerative bone
PEG-DET containing three kinds of gene, which potentially stimulate bone regeneration, was implanted to mice with appropriate scaffold, and favorable bone regeneration was observed in the scaffold.
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