| Project/Area Number |
16390527
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University (2006)
Osaka University (2004-2005) |
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Principal Investigator |
HARADA Hidemitsu Iwate Medical University, School of Dentistry, Oral Anatomy II, Professor, 歯学部, 教授 (70271210)
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| Co-Investigator(Kenkyū-buntansha) |
OHSHIMA Hayato Niigata University, Academic Assembly, Institute of Medicine and Dentistry, 医歯学系, 教授 (70251824)
ISHIZEKI Kiyoto Iwate Medical University, School of Dentistry, Oral Anatomy II, Assistant Professor, 歯学部, 講師 (50057775)
FUJIWARA Naoki Iwate Medical University, School of Dentistry, Oral Anatomy II, Assistant Professor, 歯学部, 講師 (20190100)
KAGIYA Tadayoshi Iwate Medical University, School of Dentistry, Oral Anatomy II, Assistant Professor, 歯学部, 助手 (30405774)
豊澤 悟 大阪大学, 歯学研究科, 教授 (30243249)
脇坂 聡 大阪大学, 歯学研究科, 教授 (40158598)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2005: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥7,700,000 (Direct Cost: ¥7,700,000)
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| Keywords | Fgf 10 / Fgf 9 / dental epithelial stem cells / mesenchymal stem cells / apical bud / stem cell niche / bioengineered teeth / niche / 上皮間葉相互作用 |
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| Research Abstract |
Mouse incisors are regenerative tissues, which grow continuously throughout life. We found that in the teeth, Fgf-9 plays a role of maintenance of mesenchymal cells expressing Fgf-10 and that the stem cell niche of incisors are formed by the epithelial-mesenchymal interaction via the signaling of Fgf-9 and Fgf-10. Immunostaining showed that Fgf-9 was expressed in the basal epithelium, stellate reticulum and inner enamel epithelium in the apical bud, and the expression area underlay the mesenchyme expressing Fgf-10. Recombinant Fgf-9 protein stimulated the increase of number of mesenchymal cells in a concentration-dependent manner. Annexin V staining and whole mount in situ hybridyzation using organ culture showed that recombinant Fgf-9 protein inhibited the apoptosis of mesenchymal cells of apical end and maintained the expression of Fgf-10. However, we could not success to produce bioengineered tooth germ and to culture these stem cells in the presence of only these factors.
Furthermore, we investigated the additionally factors in the culture medium and it was found that we needs FGF2 and EGF to culture the cells of the epithelial and mesenchymal stem cell compartment.
Finally, we succeeded to produce the regenerative bioengineered teeth using the dental epithelial stem cells and the mesenchymal stem cells of mouse incisors by the combination of collagen sponge and Fgf2/Egf/Fgf9/Fgf10-releasing gelatin beads. Now, we proceed to study the technical methods controlling the morphology and size of bioengineered tooth by these factors.
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