| Project/Area Number |
16405036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
AKASHI Hiroomi THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, Professor, 大学院・農学生命科学研究科, 教授 (10334327)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Yasuhiro THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, Professor, 大学院・農学生命科学研究科, 教授 (80109975)
HONDO Ryou NIPPON VETERINARY AND ANIMAL SCIENCE UNIVERSITY, DEPARTMENT OF VETERINARY PUBLIC HEALTH, Professor, 獣医畜産学部, 名誉教授 (20114639)
MORIKAWA Shigeru NATIONAL INSTITUTE OF INFECTIOUS DISEASE, DEPARTMENT OF VIROLOGY I, Laboratory head, ウイルス研究部, 室長 (00167686)
TOHYA Yukinobu THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20180119)
KYUWA Shigeru THE UNIVERSITY OF TOKYO, GRADUATE SCHOOL OF AGRICULTURAL AND LIFE SCIENCES, Associate Professor, 大学院・農学生命科学研究科, 助教授 (30177943)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2006: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Eboravirus / ELISA / epidemiological survey / immunofluorescent / anti-bat IgG rabbit serum / bat immunoglobulin / B virus / Yokose virus / ELESA法 |
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| Research Abstract |
1. Research on Eboravirus and B virus
Immunofluorescent technique using HeLa cells expressing Eboravirus nucleocapsid protein and, ELISA method with baculovirus expressed Eboravirus antigen were established to detect antibody against the virus from primates. We also established fluorometric ELISA using expressed B virus gD protein. This method could detect specific antibody against B virus from primates with no cross reactivity to human herpesviruses.
2. Research on bat immune system
Anti-bat IgG rabbit serum was produced to establish conventional ELISA method for the detection of viral antibody from bat sera. This anti-bat IgG rabbit serum reacted with IgG of Mega and Microbats tested. Moreover, we determined the nucleotide sequences of the bat CD4, IgFcRn, IFN-α subtype 1, and INF-β coding region.
3. Serological survey of bat viruses
The labeled avidin-biotin ELISA system was established to detect antibody against Yokose virus from bats. To examine the availability of this ELISA, bat serum samples collected in the Philippines and Malaysia was conducted. 2.7% of the samples from the Philippines and 19% from Malaysia had detectable antibodies. We also conducted experimental infection of Yokose virus to frugivorous bats. The results showed poor replication of the virus in bats, suggesting that bats do not seem to serve as an amplifying host for Yokose virus.
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