Activity-dependent regulation of the expression of chondroitin sulfate proteoglycan and neuronal plasticity

• Project/Area Number: 16500218
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: MIYATA Seiji Kyoto Institute of Technology, Graduate School of Science and Technology, Research Associate, 工芸科学研究科, 助手 (30243124)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,700,000); Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: Proteoglycan / Neuron / Plasticity / tPA / Phosphacan / Neurocan / Vasopressin / Perineuronal nets / コンドロイチン硫酸プロテオグリカン / オキシトシン / 脳 / Phosphacan / 細胞外マトリクス

Research Abstract

Chondroitin sulfate proteoglycans (CSPGs) are glycoproteins that carry covalently bound sulfated glycosaminoglycan chains, and more than 30 primary structures of core proteins have been elucidated. CSPGs are main components of extracellular matrix in the central nervous system. The present research program demonstrated following main results.
1) The levels of phosphacan proteoglycan was regulated with activity-dependent manner and this downregulation was mediated via proteolysis by tPA action.
2) Phosphacan was localized at axonal membrane of parallel fibers in the molecular layer and inhibited adhesion and extension of granule cells and promoted the fasciculation of parallel fibers.
3) Cultured cortical neurons were able to construct perineuronal net-like structure without glial cells. This result indicate that perineuronal net is neuronal origin.

Research Products

• [Journal Article] Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays. (2006)
  • Author(s): Ikeda, K., Nakazawa, H., Shimooka, A., Ishio, K., Miyata, S., et al.
  • Journal Title: Proteomics 6 Pages: 54-66
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Sodium-level-sensitive sodium channel Nax is expressed in glia laminate processes in the sensory circumventricular organs. (2006)
  • Author(s): Watanabe, E., Hiyama, T., Shimizu, H., Kodama, R., Hayashi, N., Miyata, S., et al.
  • Journal Title: American Journal of Physiology 290 Pages: 568-576
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays. (2006)
  • Author(s): Ikeda, K., Nakazawa, H., Shimooka, A., Miyata, S., Jarvis D.L., Hosokawa, Y., Matsumura, S., Masuhara, H., Belloncik, S., Alain, R., Nomura, N., Goshima, N., Kawai, A., Kuroita, T., Kawakami, B., Endo, Y., Morigaki, K., Mori, H.
  • Journal Title: Proteomics 6 Pages: 54-66
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sodium-level-sensitive sodium channel Nax is expressed in glial laminate processes in the sensory circumventricular organs. (2006)
  • Author(s): Watanabe, E., Hiyama, T., Shimizu, H., Kodama, R., Hayashi, N., Miyata, S., Yanagawa, Y., Obata, K., Noda, M.
  • Journal Title: American Journal of Physiology 290
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Neuronal expression of the chondroitin sulfate proteoglycans phosphacan and receptor-type protein-tyrosine phosphataseβ (2005)
  • Author(s): Hayashi, N., Miyata, S., Yamada, M., Kamei, K., Oohira, A.
  • Journal Title: Neuroscience 131 Pages: 331-348
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Heparin structures in FGF-2-dependent morphological transformation of astrocytes (2005)
  • Author(s): Nagayasu, T., Miyata, S., Hayashi, N., Kariya, Y., Takano, R., Kamei, K.
  • Journal Title: Journal of Biomedical Materials Research 74A Pages: 374-380
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Localization of receptor-type protein tyrosine phosphataseβ in the cerebral and hippocampal neurons of adult rats (2005)
  • Author(s): Hayashi, N., Oohira, A., Miyata, S.
  • Journal Title: Brain Research 1050 Pages: 163-169
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Construction of perineuronal net-like structure of cortical neurons in culture (2005)
  • Author(s): Miyata, S., Nishimura, Y., Hayashi, N., Oohira, A.
  • Journal Title: Neuroscience 136 Pages: 95-104
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Chondroitin sulfate proteoglycan phosphacan associates with parallel fiber and modulates axonal extension and fasciculation of cerebellar granule cells. (2005)
  • Author(s): Hayashi, N., Mizusaki, M., Kamei, K., Harada, T., Miyata, S.
  • Journal Title: Molecular and Cellular Neuroscience 30 Pages: 364-377
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Absorption of mulberry root urease to the hemolymph of the silkworm, Bobyx mori (2005)
  • Author(s): Kurahashi, H., Atiwetin, P., Nagaoka, S., Miyata, S., Kitajima, S., Sugimura, Y.
  • Journal Title: Journal of Insect Physiology 51 Pages: 1055-1061
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Matrix-degrading enzymes tissue plasminogen activator and matrix metalloprotease-3 in the hypothalamo-neurohypophysial system. (2005)
  • Author(s): Miyata, S., Nakatani, Y., Hayashi, N., Nakashima, T.
  • Journal Title: Brain Research 1058 Pages: 1-9
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Neuronal expression of the chondroitin sulfate proteoglycans phosphacan and receptor-type protein-tyrosine phosphates β. (2005)
  • Author(s): Hayashi, N., Miyata, S., Yamada, M., Kamei, K., Oohira, A.
  • Journal Title: Neuroscience 131 Pages: 331-348
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Heparin structures in FGF-2-dependent morphological transformation of astrocytes (2005)
  • Author(s): Nagayasu, T., Miyata, S., Hayashi, N., Kariya, Y., Takano, R., Kamei, K
  • Journal Title: Journal of Biomedical Materials Research 74A Pages: 374-380
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Localization of receptor-type protein tyrosine phosphataseζ/β in the cerebral and hippocampal neurons of adult rats. (2005)
  • Author(s): Hayashi, N., Oohira, A., Miyata, S.
  • Journal Title: Brain Research 1050 Pages: 163-169
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Absorption of mulberry root urease to the hemolymph of the silkworm, Bombyx mori. (2005)
  • Author(s): Kurahashi, H., Atiwetin, P., Nagaoka, S., Miyata, S., Kitajima, S., Sugimura, Y.
  • Journal Title: Journal of Insect Physiology 51 Pages: 1055-1061
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Constitution of perineuronal nets in cultured cortical neurons. (2005)
  • Author(s): Miyata, S., Nishimura, Y., Hayashi, N., Oohira, A.
  • Journal Title: Neuroscience 136 Pages: 95-104
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Matrix-degrading enzymes tissue plasmino activator and matrix metalloprotease-3 in hypothalamo-neurohypophysial system. (2005)
  • Author(s): Miyata, S., Nakatani, Y., Hayashi, N, Nakashima T
  • Journal Title: Brain Research 1058 Pages: 1-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Neuronal expression of the chondroitin sulfate proteoglycans phosphacan and receptor-type protein-tyrosine phosphatase β (2005)
  • Author(s): Hayashi, N., Miyata, S., Yamada, M., Kamei, K., Oohira, A.
  • Journal Title: Neuroscience 131 Pages: 331-348
• [Journal Article] Heparin structures in FGF-2-dependent morphological transformation of astrocytes (2005)
  • Author(s): Nagayasu, T., Miyata, S., Hayashi, N., Kariya, Y., Takano, R, Kamei, K.
  • Journal Title: Journal of Biomedical Materials Research 74A Pages: 374-380
• [Journal Article] Localization of receptor-type protein tyrosine phosphatase β in the Cerebral and hippocampal neurons of adult rats (2005)
  • Author(s): Hayashi, N., Oohira, A., Miyata, S.
  • Journal Title: Brain Research 1050 Pages: 163-169
• [Journal Article] Matrix-degrading enzymes tissue plasminogen activator and matrix metalloprotease-3 in the hypothalamo-neurohypophysial sytem. (2005)
  • Author(s): Miyata, S., Nakatani, Y., Hayashi, N., Nakashima, T.
  • Journal Title: Brain Research 1058 Pages: 1-9
• [Journal Article] Neuronal expression of the chondroitin sulfate proteoglycans phosphacan and receptor-type protein-tyrosine phosphatase β (2005)
  • Author(s): Hayashi N, Miyata S, Yamada M, Kamei K, Oohira A
  • Journal Title: Neuroscience 131 Pages: 331-348
• [Journal Article] Heparin structures in FGF-2-dependent morphological transformation of astrocytes (2005)
  • Author(s): Nagayama T, Miyata S, Hayashi N, Kariya Y, Takano R, Kamei K
  • Journal Title: Journal of Biomedical Materials Research In Press
• [Journal Article] Activity-dependent regulation of a chondroitin sulfate proteoglycan 6B4 phosphacan/RPTPβ in the hypothalamo-neurohypophysial system (2004)
  • Author(s): Miyata, S., Akagi, A., Hayashi, N., Watanabe, K., Oohira, A.
  • Journal Title: Brain Research 1017 Pages: 163-171
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Attenuation of glial scar formation in the injured rat brain by heparin oligosaccharides (2004)
  • Author(s): Hayashi, N., Miyata, S., Kariya, Y., Takano, R., Hara, S., Kamei, K.
  • Journal Title: Neuroscience Research 49 Pages: 19-27
  • NAID: 10023898743
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Activity-dependent regulation of a chondroitin sulfate proteoglycan 6B4 phosnhacan/RPTPβ in the hypothalamo-neurohypophysial system (2004)
  • Author(s): Miyata S, Akagi A, Hayashi N, Watanabe K, Oohira A
  • Journal Title: Brain Research 1017 Pages: 163-171
• [Journal Article] Attention of glial scar formation in the injured rat brain by heparin oligosaccharides (2004)
  • Author(s): Hayashi N, Miyata S, Kariya Y, Takano R, Hara S, Kamei K
  • Journal Title: Neuroscience Research 49 Pages: 19-27
• [Journal Article] Chondroitin sulfate proteoglycan phosphacan regulates parallel fiber pathway of developing granular neurons in the cerebellum.
  • Author(s): Hayashi, N., Mizusaki J.M., Kamei, K., Harada, T., Miyata, S.
  • Journal Title: Molecular and Cellular Neuroscience 30 Pages: 364-377
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays.
  • Author(s): Ikeda, K., nakazawa, H., Shimooka, A., Ishio, K., Miyata, S., et al.
  • Journal Title: Proteomics (In press)
• [Journal Article] Sodium-level-sensitive sodium channel Nax is expressed in glia laminate processes in the sensory circumventricular organs.
  • Author(s): Watanabe, E., hiyama, T., Shimizu, H., Kodama, R., hayashi, N., Miyata, S., et al.
  • Journal Title: American Journal of Physiology (In press)