Cell biological study of the RANDAM-2 high positive cells and RANDAM-2 low/negative cells in mouse central nervous system.

• Project/Area Number: 16500252
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Ohu University (2005); Tokyo Metropolitan Organization for Medical Research (2004)
• Principal Investigator: KOTANI Masaharu Ohu University, Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10195737)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,700,000); Fiscal Year 2005: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: Mouse neurospheres / neural stem cells / cell membrane antigens / RANDAM-2 / FACS analysis / FACS analysis / 神経幹細胞 / 神経細胞分化 / 中枢神経系 / マウス

Research Abstract

RANDAM-2, a type-I transmembrane antigen constitutively expressed on the excitatory neuronal cell lineage during mouse neurogenesis, shows the highest expression level between embryonic day 8.5 (E8.5) and E10.5. As the period well overlaps with the proliferating stages of neural stem cells (NSCs), it is conceivable that RANDAM-2 is involved in the proliferation and/or the resulting neural differentiation. In this paper, we show that NSCs and NPCs can be isolated as RANDAM-2^<high+> and RANDAM-2^<low+/-> cells, respectively, by positive selection with a monoclonal antibody specific to RANDAM-2. The isolated RANDAM-2^<high+> cells had the characteristics as the highly self-renewal capability and potential for multilineage differentiation into neural cells. In contrast, almost all of the RANDAM-2^<low+/-> cells exhibited not only the extremely low self-renewability but the differentiation capability restricted to neurons. These two cell populations also differed from each other in terms of the expression level of molecules associated with neural differentiation. These findings demonstrate that RANDAM-2 can be regarded as a useful marker for isolation of the NSCs, and suggest that its high expression takes part in the retention of the neural cells within the innate properties as NSCs.

Research Products

• [Journal Article] Distal myopathy with rimmed vacuoles : Impaiered-Ο-glycan formation in sarcoemmal glycoprooteins. (2005)
  • Author(s): Tajima, Y., Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M.et al.
  • Journal Title: American Journal of Pathology 166 Pages: 1121-1130
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment of immortalized Schwann cells from Sandhoff mice and corrective effect of recombinant human beta-hexosaminidase A on the accumulated GM2 ganglioside. (2005)
  • Author(s): Murata, M., Motani, M.et al.
  • Journal Title: Journal of Human Genetics 50 Pages: 460-467
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An asymptomatic heterozygous female with Fabry disease : Implication for enzyme replacement therapy. (2005)
  • Author(s): Inagaki, S., Migita, M., Hayakawa, M., fujita, A., Yoshida, J., Ishizaki, M., Kotani, M.et al.
  • Journal Title: Journal of Nippon Medical School 72 Pages: 687-690
  • NAID: 130004146823
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Distal myopathy with rimmed vacuoles : Impaired O-glycan formation in sarcolemmal glycoproteins. (2005)
  • Author(s): Tajima, Y, Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M., et al.
  • Journal Title: American Journal of Pathology 166(4) Pages: 1121-1130
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Establishment of immortalized Schwann cells from Sandhoff mice and corrective effect of recombinant human beta-hexosaminidase A on the accumulated GM2 ganglioside. (2005)
  • Author(s): Murata, M., Kotani, M., et al.
  • Journal Title: Journal of Human Genetics 50 Pages: 460-467
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An asymptomatic heterozygous female with Fabry disease : Implication for enzyme replacement therapy. (2005)
  • Author(s): Inagaki, S., Migita, M., Hayakawa, M., Fujita, A., Yoshida, J., Ishizaki, M., Kotani, M., et al.
  • Journal Title: Journal of Nippon Medical School 72(6) Pages: 687-690
  • NAID: 130004146823
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Distal myopathy with rimmed vacuoles : Impaired O-glycan formation in sarcolemmal glycoproteins. (2005)
  • Author(s): Tajima, Y., Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M., et al.
  • Journal Title: American Journal of Pathology 166 Pages: 1121-1130
• [Journal Article] An asymptomatic heterozygous female with Fabry disease : Implication for enzyme replacement therapy. (2005)
  • Author(s): Inagaki, S., Migita, M., Hayakawa, M., Fujita, A., Yoshida, J., Ishizaki, M., Kotani, M., et al.
  • Journal Title: Journal of Nippon Medical School 72 Pages: 687-690
  • NAID: 130004146823
• [Journal Article] Cytochemical and biochemical detection of intracellularly accumulate sialyl glycoconjugates in sialidosis and galactosialidosis fibroblasts with Macckia amurensis. (2004)
  • Author(s): Motegi, A., Fujimoto, J., Kotani, N.et al.
  • Journal Title: Clinica Chimica Acta 344 Pages: 131-135
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] ALK receptor tyrosine kinase promotes cell growth and neurite outgrowth. (2004)
  • Author(s): Motegi, A., Fujimoto, J., Kotani, N.et al.
  • Journal Title: Journal of Cell Science 117 Pages: 3319-3329
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Reversibility versus persistence of GPIIb/IIIa blocker-induced conformational change of GP_IIb/III_a (aIIbβ3,CD41/CD61) (2004)
  • Author(s): Schwarz, M., Katagiri, Y., Kotani, M.et al.
  • Journal Title: Journal of Pharmacology and Experimental Therapeutics 308 Pages: 1002-1011
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cytochemical and biochemical detection of intracellularly accumulate sialyl glycoconjugates in sialidosis and galactosialidosis fibroblasts with Macckia amurensis. (2004)
  • Author(s): Kotani, M., Yamada, H., Sakuraba, H.
  • Journal Title: Clinica Chimica Acta 344 Pages: 131-135
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] ALK receptor tyrosine kinase promotes cell growth and neurite outgrowth. (2004)
  • Author(s): Motegi, A., Fujimoto, J., Kotani, M, Sakuraba, H., Yamamoto, T.
  • Journal Title: Journal of Cell Science 117 Pages: 3319-3329
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reversibility versus persistence of GPIIb/IIIa blocker-induced conformational change of GP_<IIb>/III_a (αIIbβ3, CD41/CD61). (2004)
  • Author(s): Schwarz, M., Katagiri, Y, Kotani, M., et al.
  • Journal Title: Journal of Pharmacology and Experimental Therapeutics 308(3) Pages: 1002-1011
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cytochemical and biochemical detection of intracellularly accumulated sialyl glycoconjugates in Sialidosis and galactosialidosis fibroblasts with Maackia amurensis. (2004)
  • Author(s): Kotani, M., Yamada, H., Sakuraba, H.
  • Journal Title: Clinical and Chimical Acta 344 Pages: 131-135
• [Journal Article] Structure and immunocytochemical studies on α-N-acetylgalactosaminidase deficiency (shindler/Kanzaki desease). (2004)
  • Author(s): Sakuraba, H., Matsuzawa, F., Aikawa, S., Doi, Y., Kotani, M., et al.
  • Journal Title: Journal Human Genetics 49 Pages: 1-8
• [Journal Article] ALK receptor tyrosine kinase promotes cell growth and neurite outgrowth as revealed by an agonist anti-ALK monoclonal antibody. (2004)
  • Author(s): Motegi, A., Fujimoto, J., Kotani, M., et al.
  • Journal Title: Journal cell Science 117 Pages: 3319-3329
• [Journal Article] Clinical and biochemical studies on a Japanese Salla disease case associated with a renal disorder. (2004)
  • Author(s): Ishiwari, K., Kotani, M., et al.
  • Journal Title: Journal Human Genetics 49 Pages: 656-663
• [Journal Article] Distal myopathy with rimmed vacuoles : Impaired O-glycan formation in saroolemmal glycoproteins. (2004)
  • Author(s): Tajima, Y., Uyama, E., Go, S., Sato, C., Tao, N., Kotani, M., et al.
  • Journal Title: American Journal of Pathology (in press)