Development of the gene deletion model rats by random mutagenesis.
| Project/Area Number |
16500282
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | National Institute of Radiological Sciences (NIRS) |
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Principal Investigator |
HARADA Yoshinobu NIRS, Molecular Imaging Center, 分子イメージング研究センター, 研究員 (90192707)
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| Co-Investigator(Kenkyū-buntansha) |
SUN Xue Zhi NIRS, Research Center for Radiation Protection, 放射線防護研究センター, 研究員 (00284323)
KITO Seiji NIRS, Research Center for Radiation Protection, 放射線防護研究センター, 研究員 (20311376)
SAGARA Masashi NIRS, Molecular Imaging Center, 分子イメージング研究センター, 研究員 (30291107)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | rat / chlorambucil / mutagenesis / gene deletion / model animal / 遺伝子欠損 / モデル運動 / マイクロアレイ |
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| Research Abstract |
Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 2.5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. We obtained several dwarfish rats from BN and F344 strans. Dwarfish rats whose sizes of the body were about 30% of normal rats were also observed. We also obtained a mutant rat with fiblosarcoma from the BN strain. This fiblosarcoma has grown up to 5cm or more in the diameter within 2 weeks. Unfortunately, we have not determined the genome region of delition of this rat by quontitative PCR analysis. Future experiments will focus on searching this genome region of delition by microarray or BAC array analyses.
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Report
(3 results)
Research Products
(28 results)
