RNA LEGO : Construction of functional RNA nanostructures
| Project/Area Number |
16510082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tokyo Gakugei University |
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Principal Investigator |
HARADA Kazuo Tokyo Gakugei University, Faculty of Education, Associate Professor, 教育学部, 助教授 (00301169)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | RNA folding / loop-loop interaction / RNA nanostructures / RNA dendrimers / thermal isomerization / peptide-binding / triple-helical scaffold / loop-loop (kissing) interaction / RNA nanostructure / RNA dendrimer |
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| Research Abstract |
We have previously shown that RNA substrates consisting of two stem-loops connected by a short linker can be used to construct specific circular or linear self-assemblies through loop-loop interactions. We also showed that by adjusting the strength of the loop-loop interaction, the assembly and disassembly of the RNA structures could be controlled in a magnesium-dependent manner (Horiya et al., Chem.＆ Biol., 2003).
During this project term, we obtained the following results:
1) We showed that RNA substrates consisting of three stem-loops could be used to construct dendrimeric assemblies (Fujiya and Harada, Nucleic Acids Symp.Ser., 2004).
2) We demonstrated that the interconversion between circular and linear RNA assemblies could be regulated by an external substrate such as magnesium ion (Ohmori et al., Nucleic Acids Symp.Ser., 2005).
3) We showed that circular RNA assemblies can be efficiently isomerized to a thermodynamically stable structure. Using this structural isomerization as a functional switch, RNA substrates that assemble into circular dimers through loop-loop interactions, and isomerizes to form peptide binding sites and catalytic domains were designed (Li et al., J.Am.Chem.Soc., 2006).
4) We showed that thermal isomerization can be used as a switch to construct complex nanostructures. This was accomplished by designing RNA loop-loop complexes that upon thermal isomerization form 5'- and 3'-single stranded regions complementary to each other. Such substrates can be used to form linear and two-dimensional assemblies (Kunimoto et al., in preparation).
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] RNA technology2006
Author(s)
K.Harada
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Journal Title
Functional non-coding RNAs (G.Kawai, A.Kanai, eds.), Kubapro
Pages: 221-236
Description
「研究成果報告書概要(欧文)」より
Related Report
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