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Atomic Force Acoustic Microscopy for Imaging and Characterizing Biological Molecules

Research Project

Project/Area Number 16560058
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Materials/Mechanics of materials
Research InstitutionAkita University

Principal Investigator

MURAOKA Mikio  Akita University, Faculty of Engineering and Resource Science, Associate Professor, 工学資源学部, 助教授 (50190872)

Co-Investigator(Kenkyū-buntansha) OKUYAMA Eiki  Akita University, Faculty of Engineering and Resource Science, Associate Professor, 工学資源学部, 助教授 (80177188)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsAtomic force acoustic microscopy / DNA molecules / DNA chips for genetic diagnoses / Adhesion force / Energy release rate / Concentrated-mass cantilever / Shear force control / Silicon tip / 高感度化技術 / 付着エネルギー / 弾性計測
Research Abstract

The purpose of this study is to develop atomic force acoustic microscopy for detecting mechanical properties of biological molecules together with imaging the molecules. The results are summarized as follows :
1. Immobilization of probe DNA molecules on chips is important in a view point of reliability of genetic diagnoses using DNA chips. Optimization of process of DNA chips requires evaluation of DNA chips for how strong the molecules are fixed on a substrate. As a preliminary research, we developed a method of measuring the bond strength between a DNA molecule and a substrate. Hydrogen bonds stick DNA molecules to silicon tips in water. Therefore silicon tips can pick up and tear the molecules from plastic substrates such polycarbonate chips. The force curve revealed long-range attractive force with roughly constant values at retraction of a cantilever from the substrate. The long-range force reflects the tearing process of molecules. We introduced an energy release rate for the tearing process to determine the adhesion energy per a unit length of a DNA molecule. The analysis clarified the chemical bonding between a polycarbonate substrate and a DNA molecule is mainly hydrogen bonding.
2. In contrast to the measurement in water (result 1), the adhesion between a silicone tip and a DNA molecule on a polycarbonate substrate is weak in air. DNA molecules shield the strong attractive force between a silicon tip and a polycarbonate. This finding can be used for imaging a DNA molecule on a polycarbonate substrate with a surface roughness as a candidate of commercial DNA chips.
3. We developed atomic force acoustic microscopy (AFAM) under a shear force control for imaging of biological molecules. A rod-like gold rod is utilized as a concentrated-mass (CM) for a cantilever. The CM cantilever is efficiently excited to produce a shear force mode of vibration.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005

All Journal Article (17 results)

  • [Journal Article] シアフォース制御ナノ加工用の集中質量型カンチレバー2006

    • Author(s)
      石川広美
    • Journal Title

      日本機械学会東北支部第41期総会・講演会講演論文集

      Pages: 131-132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Concentrated-Mass Cantilever for Nano-Machining Under Detection of Shear Force2006

    • Author(s)
      Hiromi ISHIKAWA
    • Journal Title

      Proc.2005 Annual Meeting of JSME Tohoku Regional Affiliate No.2006-1

      Pages: 131-132

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] シアフォース制御ナノ加工用の集中質量型カンチレバー2006

    • Author(s)
      石川 広美, 伊藤 卓将, 村岡 幹夫
    • Journal Title

      日本機械学会東北支部第41期総会・講演会講演論文集 NO.2006-1

      Pages: 131-132

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 遺伝子診断チップ上DNA分子のAFMによる付着エネルギー評価2005

    • Author(s)
      荒井俊昭
    • Journal Title

      日本機械学会東北学生会第35回講演会講演論文集

      Pages: 3-4

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sensitivity-enhanced atomic force acoustic microscopy with concentrated-mass cantilevers2005

    • Author(s)
      Mikio Muraoka
    • Journal Title

      Nanotechnology 16

      Pages: 542-550

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 遺伝子診断チップ上DNA分子のキャラクタリゼーション(付着エネルギーと面密度)2005

    • Author(s)
      加藤 勝
    • Journal Title

      日本機械学会M&M2005材料力学カンファレンス講演論文集

      Pages: 599-600

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 原子間力超音波顕微鏡によるDLCナノ薄膜の弾性率評価2005

    • Author(s)
      村岡幹夫
    • Journal Title

      日本機械学会2005年度年次大会講演論文集

      Pages: 313-314

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Measurement of Thin Film Elasticity Using Nanoscopic Contact Resonance of a Flat Tip in Sensitivity-Enhanced Atomic Force Acoustic Microscopy2005

    • Author(s)
      Mikio Muraoka
    • Journal Title

      Proc. 2nd JSME/ASME Int. Conf. on Materials and Processing, Seatle (CD-ROM)

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Atomic-Force Microscopic Evaluation of DNA Molecules on Diagnostic Chip for the Adhesion Energy2005

    • Author(s)
      Toshinori ARAI
    • Journal Title

      Proc.2005 Annual Meeting of JSME Tohoku Regional Student Division

      Pages: 3-4

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Sensitivity-Enhanced Atomic Force Acoustic Microscopy with Concentrated-Mass Cantilevers2005

    • Author(s)
      Mikio MURAOKA
    • Journal Title

      Nanotechnology Vol.16

      Pages: 542-550

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of DNA Molecules Adsorbed on Diagnostic Chips by Atomic Force Microscopy (Adhesion Energy and Surface Density)2005

    • Author(s)
      Masaru KATO
    • Journal Title

      Proc.2005 Annual Meeting of the JSME/MMD No.05-9

      Pages: 599-600

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] DLC-Thin-Film Characterization by Sensitivity-Enhanced Atomic Force Acoustic Microscopy2005

    • Author(s)
      Mikio MURAOKA
    • Journal Title

      Proc.Mechanical Engineering Congress No.05-1

      Pages: 313-314

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Measurement of Thin Film Elasticity Using Nanoscopic Contact Resonance of a Flat Tip in Sensitivity-Enhanced Atomic Force Acoustic Microscopy2005

    • Author(s)
      Mikio MURAOKA
    • Journal Title

      Proc.2nd JSME/ASME Int.Conf.on Materials and Processing Paper No.SNS-02(CD-ROM)

      Pages: 1-4

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 遺伝子診断チップ上DNA分子のキャラクタリゼーション(付着エネルギーと面密度)2005

    • Author(s)
      加藤 勝, 村岡 幹夫, 赤上 陽一
    • Journal Title

      日本機械学会M&M2005材料力学カンファレンス講演論文集 No.05-9

      Pages: 599-600

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 原子間力超音波顕微鏡によるDLCナノ薄膜の弾性率評価2005

    • Author(s)
      村岡 幹夫, 小松 慎司, 泉田 福典
    • Journal Title

      日本機械学会2005年度年次大会講演論文集 1・05-1

      Pages: 313-314

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Measurement of Thin Film Elasticity Using Nanoscopic Contact Resonance of a Flat Tip in Sensitivity-Enhanced Atomic Force Acoustic Microscopy2005

    • Author(s)
      M.Muraoka, S.Komatsu, F.Izumida
    • Journal Title

      Proc.2nd JSME/ASME Int.Conf.on Materials and Processing, Seatle, CD-ROM Paper No.SMS-02

      Pages: 1-4

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 遺伝子診断チップ上DNA分子のAFMによる付着エネルギー評価2005

    • Author(s)
      荒井俊昭, 村岡幹夫
    • Journal Title

      日本機械学会東北学生会第35回講演会講演論文集

      Pages: 3-4

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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