Design of nano-particle type drug delivery system with thermosensitive bioelastic polypeptides by radiation crosslinking
| Project/Area Number |
16560736
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nuclear engineering
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
FURUTA Masakazu Osaka Prefecture University, Graduate School of Sciences, Associate Professor, 理学系研究科, 助教授 (40181458)
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| Co-Investigator(Kenkyū-buntansha) |
OKUDA Shuichi Osaka Prefecture University, Frontier Science Innovation Center, Professor, 産学官連携機構, 教授 (00142175)
HARA Masayuki Osaka Prefecture University, Graduate School of Sciences, Professor, 理学系研究科, 教授 (50344172)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | nano material / thermosensitivity / polypeptide / LCST / radiation crosslinking / エラスチン / ナノ粒子 / DDS |
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| Research Abstract |
Elastic protein-based polymers were prepared by recombinant DNA technology and based on the GVGVP repeat amino acid sequence of tropoelastin. Various heating condition was examined for aqueous solutions of the polypeptides to obtain an appropriate nano-particle suspensions. Under the specified condition of "heat shock" ; dropping the concentrated solution into the hot water (40℃), they can be □-irradiation cross-linked to form nano-particles that pass through a 0.22nm filter and exhibit diameters of less than ca.200nm and a narrow size distribution. Yield of the □-cross-linked nano-particles increases with increasing dose above 8.5 kGy but is insensitive to dose rate. We confirmed the particles by TEM. GPC and SDS-PAGE analyses strongly suggested that gamma-rays caused mostly crosslinking within the particles. Time-release profiles for Biebrich Scarlet Red indicated continuous release of the dye for more than 2 weeks. Thus, this controlled release device can deliver drugs to sites accessible by small caliber hypodermic syringes.
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Report
(3 results)
Research Products
(12 results)
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[Book] Elastic Protein-Based Elements of Basic Sciences, Controlled Release, and Biocompatibility, Tissue Engineering and Novel Delivery Systems(Marcel Dekker)2004
Author(s)
D.W.Urry, T.C.Woods, L.C.Hayes, J.Xu, D.T.McPherson, T.M.Parker, M.Iwama, M.Furuta, T.Hayashi, M.Murata
Publisher
Inc.New York
Description
「研究成果報告書概要(欧文)」より
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