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Isotypes of multilocalizing proteins from the cytoskeleton and their roles in differentiation and growth of pea seedlings.

Research Project

Project/Area Number 16570034
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionEhime University

Principal Investigator

ABE Shunnosuke  Ehime University, Faculty of Agriculture, professor, 農学部, 教授 (40167937)

Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsapyrase / Tudor / differentiation / Lateral root formation / sub-cellular localization / immunohistochemistry / translation / cytoskeleton / 4SNc-Tudor / 発芽 / マメ類 / 誘導酵素 / アピラーゼ / SN4TDR / 吸水 / 遺伝子発現 / タンパク質 / 分裂酵母
Research Abstract

Expression, localization, and molecular structure of the heparin binding protein SN4TDR and apy1 from the pea cytoskeleton were studied. Both SN4TDR and apy1 were localized in the cytoplasm in pea seedling tissues and also induced in the primodium for lateral root formation. In some active cells, apy1 was also localized in nuclei. However, expression of SN4TDR was induced during imbibition and kept constant after germination as in tub1, while apy1 was induced 16 h after germination. Localization of these proteins in the cytoplasm were proved by immunohistochemistry in pea seedlings. When with the full SN4TDR was fused with XFP and expressed in S.pombe, the protein was proved to localize in cytoplasm, leaving nuclei unstained throughout the life cycle (vegetative, mating, and sporulation. Deletion experiments of the YFP construct in S.pombe implied the presence of nuclear exclusion signal (NES) with less than 50 aa long in SN4TDR. Yeast two hybridization followed by immunoprecipitation.proposed the tubulin binding subunit of translation initiation factor, moe1 and ribosomal protein L22 potent as candidates for association of SN4TDR in vivo. Phosphorylation of apy1 was also proved.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (17 results)

All 2007 2006 2005 2004

All Journal Article (15 results) Book (2 results)

  • [Journal Article] Molecular Basis of Ethylene Signal Transduction and Control of Abscission of Flowers in Delphinium2006

    • Author(s)
      Shunnosuke Abe, Satoshi Kuroda, Yukio Hirose, Eric Davies.
    • Journal Title

      Floriculture, Ornamental and Plant Biotechnology : Advances and Topical Issues Chapter 13(ed: Jaime A.Teixeira da Silva)(Global Science Books Ltd. UK) Volume 1

      Pages: 109-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural analysis of cDNAs coding for 4SNc-Tudor domain protein from fish and their expression in yellowtail organs2005

    • Author(s)
      阿部俊之助ほか
    • Journal Title

      Marine Biotechnology 7

      Pages: 677-686

    • NAID

      10018624518

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and identification of 3-methylcrotonyl CoA carboxylase cDNAs and pyruvate carboxylase, and their expression in red seabream, Pagrus major organs2005

    • Author(s)
      阿部俊之助ほか
    • Journal Title

      Marine Biotechnology 6

      Pages: 527-540

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural analysis of cDNAs coding for 4SNc-Tudor domain protein from fish and their expression in yellowtail organs2005

    • Author(s)
      Shunnosuke Abe, Pi-Lin Wang, Fuminori Takahashi, Eiji Sasaki
    • Journal Title

      Marine Biotechnology 7

      Pages: 677-686

    • NAID

      10018624518

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and identification of 3-methylcrotonyl CoA carboxylase cDNAs and pyruvate carboxylase, and their expression in red seabream, Pagrus major organs2005

    • Author(s)
      Shunnosuke Abe, Chhoun Chamnan, Kenichi Miyamoto, Yasutaka Minamino, Makoto Nouda
    • Journal Title

      Marine Biotechnology 6(6)

      Pages: 527-540

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Role of 4SNc-Tudor domain protein in protein synthesis in plants : functional analysis using a model system, Schizosaccharomyces pombe2005

    • Author(s)
      Shunnosuke Abe
    • Journal Title

      Supplement to Plant Physiology : Abstract for Plant Biology 2005 July 16-20,2005,Seattle, Washington, USA

      Pages: 501-501

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of apyrase(APY1) and Tudor protein(SN4TDR) during early stages of germination of peas(Pisum sativum L.var.Alaska) in the dark2005

    • Author(s)
      Shunnosuke Abe
    • Journal Title

      Supplement to Plant Physiology : Abstract for Plant Biology 2005 July 16-20,2005, Seattle, Washington, USA

      Pages: 538-538

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination2004

    • Author(s)
      阿部俊之助ほか
    • Journal Title

      Acta Physiol Plant 25

      Pages: 67-74

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antioxidant Activity of Triticale Caryopses and Embryos Extracts2004

    • Author(s)
      阿部俊之助ほか
    • Journal Title

      Food Science and Biotechnology

      Pages: 421-424

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Co-expression of an ethylene receptor gene, ERS1, and ethylene signaling regulator gene, CTR1, in Delphinium during abscission of florets2004

    • Author(s)
      阿部俊之助ほか
    • Journal Title

      Plant Physiology and Biochemistry 42

      Pages: 745-751

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Co-expression of an ethylene receptor gene, ERS1, and ethylene signaling regulator gene, CTR1, in Delphinium during abscission of florets;2004

    • Author(s)
      Satoshi Kuroda, Yukio Hirose, Masaya Shiraishi, Eric Davies, Shunnosuke Abe.
    • Journal Title

      Plant Physiology and Biochemistry 42

      Pages: 745-751

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antioxidant Activity of Triticale Caryopses and Embryos Extracts2004

    • Author(s)
      Magdalena Karamac, Ryszard Amarowicz, Stanislaw Weidner, Shunnosuke Abe, Shahidi, S.
    • Journal Title

      Food Science and Biotechnology 13(4)

      Pages: 421-424

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination2004

    • Author(s)
      Kosowska, M., Ewa Fraczek, Ryszard Amarowicz, Magdalena Karamac, Shunnosuke Abe, Stanislaw Weidner.
    • Journal Title

      Acta Physiologiae Plantarum 26(1)

      Pages: 67-74

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Water-deficit-induced changes in cytoskeleton-bound and other polysomal populations in embryonic tissue during triticale caryopsis germination2004

    • Author(s)
      阿部 俊之助
    • Journal Title

      Acta Physiol Plant 26

      Pages: 67-74

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Role of 4SNc-Tudor domain protein in protein synthesis in plants : functional analysis using a model system, Schizosaccharomyces pombe2004

    • Author(s)
      阿部 俊之助
    • Journal Title

      World Rice Research Conference 2004 Abstracts

    • Related Report
      2004 Annual Research Report
  • [Book] Molecular Basis of Ethylene Signal Transduction and Control of Abscission of Flowersin Delphinium : In Floriculture2007

    • Author(s)
      阿部俊之助
    • Total Pages
      645
    • Publisher
      Syon Lane, Isleworth, UK,
    • Related Report
      2006 Annual Research Report
  • [Book] Molecular Basis of Ethylene Signal Transduction and Control of Abscission of Flowers in Delphinium2006

    • Author(s)
      Shunnosuke Abe
    • Publisher
      Global Science Books Ltd., UK
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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