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Mechanism and role of phagocytosis of unwanted cells

Research Project

Project/Area Number 16570112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionKanazawa University

Principal Investigator

SHIRATSUCHI Akiko  Kanazawa Univ., Grad.Sch.Med.Sci., Assistant Professor, 医学系研究科, 講師 (90303297)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsphagocytosis / innate immuniti / phosphatidylserine / spermatogenesis / influenza virus / scavenger receptor / insect immunity
Research Abstract

1) Mechanism and role of SR-BI-mediated apoptotic spermatogenic cells by Sertoli cells in the testis
After binding of phosphatidylserine on the surface of apoptotic cells, SR-BI in Sertoli cells induces phhosphorylation of MAP kinase p38 and ERKI/II. In the presence of inhibitors for p38-pathway and ERK-pathway, phagocytosis of apoptotic cells by Sertoli cells was inhibited greatly. These results showed that SR-BI, when it binds to phosphatidylserine, transmits signals to activate MAP kinase pathway, which leads to the induction of the engulfment of phosphatidylserine-exposing apoptotic cells by phagocytic cells.
2) Role of phagocytosis of ineluenza virus-infected cells by phagocytes
In the lung of influenza virus-infected mice, virus-infected cells were shown in both neutrophils and alveolar macrophages. Administration of the phagocytosis inhibitors into the lung caused both the lethality in mice and the extent of inflammation in the lung were augmented in those mice. These results suggest that phagocytosis of virus-infected cells helps suppress the progress of influenza in mice.
3) Regulation of phagosome-lysosome fusion
MAP kinase p38-pathway and ERK-pathway were activated in macrophages after incubation with apoptotic cells, and the activity seemed to be needed to phagosome-lysosome fusion in macrophages.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (31 results)

All 2006 2005 2004

All Journal Article (29 results) Book (2 results)

  • [Journal Article] Selective expression of the scaffold protein JSAP1 in spermatogonia and spermatocytes.2006

    • Author(s)
      Bayarsaikhan, M., Shiratsuchi, A., et al.
    • Journal Title

      Reproduction 131

      Pages: 711-719

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Involvement of COX-1 and up-regulated prostaglandin E synthases in phosphatidylserine liposome-induced prostaglandin E2 production by microslia.2006

    • Author(s)
      Zhang, J., Shiratsuchi, A., Nakanishi, Y., Nakanishi, H.et al.
    • Journal Title

      J.Neuroimmunol. 172

      Pages: 112-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Selective expression of the scaffold protein JSAP1 in spermatogonia and spermatocytes.2006

    • Author(s)
      Bayarsaikhan, M., Shiratsuchi, A., Gantulga, D., Nakanishi, Y., Yoshioka, K.
    • Journal Title

      Reproduction 131

      Pages: 711-719

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Involvement of COX-1 and up-regulated prostaglandin E synthases in phosphatidylserine liposome-induced prostaglandin E2 production by microglia.2006

    • Author(s)
      Zhang, J., Fujii, S., Wu, Z., Hashioka, S., Tanaka, Y., Shiratsuchi, A., Nakanishi, Y., Nakanishi, H.
    • Journal Title

      J.Neuroimmunol. 172

      Pages: 112-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Selective expression of the scaffold protein JSAP1 in spermatogonia and spermatocytes.2006

    • Author(s)
      Bayarsaikhan, M., Shiratsuchi, A., et al.
    • Journal Title

      Reproduction (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Involvement of COX-1 and up-regulated prostaglandin E synthases in phosphatidylserine liposome-induced prostaglandin E2 production by microglia.2006

    • Author(s)
      Zhang, J., Shiratsuchi, A., Nakanishi, Y., Nakanishi, H.et al.
    • Journal Title

      J. Neuroimmunol. (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Augumentation of fatality of influenza in mice by inhibition of phagocytosis.2005

    • Author(s)
      Watanabe, Y., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      Biochem.Biophys.Res.Commun 337

      Pages: 881-886

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Isolation of Drosophila gene coding for a protein containinga novel phosphatidylserine-binding motif.2005

    • Author(s)
      Nakai, Y., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      J.Biochem. 137

      Pages: 593-599

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phosphatidylserine- and integrin-mediated phagocytosis of apoptotic rat luteal cells by macrophages.2005

    • Author(s)
      Kato, S., Shiratsuchi, A., Nakanishi, Y.
    • Journal Title

      Dev.Growth Differ. 47

      Pages: 153-161

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Externalization and recognition by macrophages of large subunit of eukaryotic translation initiation factor 3 in apoptotic cells.2005

    • Author(s)
      Nakai, Y., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      Exp.Cell Res. 309

      Pages: 137-148

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Distinct localization of lipid rafts and externalized phosphatidylserine at the surface of apoptotic cells.2005

    • Author(s)
      Ishii, H., Shiratsuchi.A., Nakanishi, Y.et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 327

      Pages: 94-99

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] In vivo analysis of phagocytosis of apoptotic cells by testicular Sertoli cells.2005

    • Author(s)
      Nakagawa, A., Shiratsuchi.A., Nakanishi, Y.et al.
    • Journal Title

      Mol.Reprod.Dev. 48

      Pages: 875-881

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Augumentation of fatality of influenza in mice by inhibition of phagocytosis.2005

    • Author(s)
      Watanabe, Y., Hashimoto, Y., Shiratsuchi, A., Takizawa, T., Nakanishi, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 337

      Pages: 881-886

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Externalization and recognition by macrophages of large subunit of eukaryotic translation initiation factor 3 in apoptotic cells.2005

    • Author(s)
      Nakai, Y., Shiratsuchi, A., Manaka, J., Nakayama, H., Takio, K., Zhang, J., Nakanishi, Y.
    • Journal Title

      Exp.Cell Res. 309

      Pages: 137-148

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation of Drosophila gene coding for a protein containinga novel phosphatidylserine-binding motif..2005

    • Author(s)
      Nakai, Y., Nomura, Y., Sato., T., Shiratsuchi, A., Nakanishi, Y.
    • Journal Title

      J.Biochem. 137

      Pages: 593-599

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phosphatidylserine-and integrin-mediated phagocytosis of apoptotic rat luteal cells by macrophages.2005

    • Author(s)
      Kato, S., Shiratsuchi, A., Nakanishi, Y.
    • Journal Title

      Dev.Growth Differ. 47

      Pages: 153-161

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] In vivo analysis of phagocytosis of apoptotic cells by testicular Sertoli cells.2005

    • Author(s)
      Nakagawa, A., Shiratsuchi, A., Nakanishi, Y.
    • Journal Title

      Mol.Reprod.Dev. 71

      Pages: 166-177

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Distinct localization of lipid rafts and externalized phosphatidylserine at the surface of apoptotic cells.2005

    • Author(s)
      Ishii, H., Mori, T., Shiratsuchi, A., Nakai, Y., Shimada, Y., Ohno-Iwashita, Y., Nakanishi, Y.
    • Journal Title

      Biochem.Biophys.Res.Commun. 327

      Pages: 94-99

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation of Drosophila gene coding for a protein containinga novel phosphatidylserine-binding motif..2005

    • Author(s)
      Nakai, Y., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      J.Biochem. 137

      Pages: 593-599

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Distinct localization of lipid rafts and externalized phosphatidylserine at the surface of apoptotic cells.2005

    • Author(s)
      Ishii, H., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] In vivo analysis of phagocytosis of apoptotic cells by testicular Sertoli cells.2005

    • Author(s)
      Nakagawa, A., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      Mol.Reprod.Dev. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibitory effect of Toll like receptor 4 on fusion between phagosomes and endosomes/lysosomes in macrophages.2004

    • Author(s)
      Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      J.Immunol. 172

      Pages: 2039-2047

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Expression and function of class B scavenger receptor type I on both apical and basolateral sides of the plasma membrane of polarized testicular Sertoli cells of the rat.2004

    • Author(s)
      Nakagawa, A., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      Develop.Growth Differ. 46

      Pages: 283-298

    • NAID

      10013279608

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Draper-mediated and phosphatidylserine-independent phagocytosis of apoptotic cells by Drosophila hemocytes/macrophages.2004

    • Author(s)
      Manaka, J., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 48466-48476

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Stimulation of phagocytosis of influenza virus-infected cells through surface desialylation of macrophages by viral neuraminidase.2004

    • Author(s)
      Watanabe, Y., Shiratsuchi, A., Nakanishi, Y.et al.
    • Journal Title

      Microbiol.Immunol 48

      Pages: 875-881

    • NAID

      10014023511

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Stimulation of phagocytosis of influenza virus-infected cells through surface desialylation of macrophages by viral neuraminidase.2004

    • Author(s)
      Watanabe, Y., Shiratsuchi, A., Shimizu, K., Takenori Takizawa, T., Nakanishi, Y.
    • Journal Title

      Microbiol.Immunol. 48

      Pages: 875-881

    • NAID

      10014023511

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Draper-mediated and phosphatidylserine-independent phagocytosis of apoptotic cells by Drosophila hemocytes/macrophages.2004

    • Author(s)
      Manaka, J., Kuraishi, T., Shiratsuchi, A., Nakai, Y., Higashida, H., Henson, P., Nakanishi, Y.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 48466-48476

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and function of class B scavenger receptor type I on both apical and basolateral sides of the plasma membrane of polarized testicular Sertoli cells of the rat.2004

    • Author(s)
      Nakagawa, A., Nagaosa, K., Hirose, T., Tsuda, K., Hasegawa, K., Shiratsuchi, A., Nakanishi, Y.
    • Journal Title

      Develop.Growth Differ. 46

      Pages: 283-298

    • NAID

      10013279608

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Inhibitory effect of Toll like receptor 4 on fusion between phagosomes and endosomes/lysosomes in macrophages.2004

    • Author(s)
      Shiratsuchi, A., Watanabe, I., Takeuchi, O., Akira, S., Nakanishi, Y.
    • Journal Title

      J.Immunol. 172

      Pages: 2039-2047

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 薬学生のための細胞生物学2005

    • Author(s)
      堅田利明, 白土明子, 中西義信他8名
    • Total Pages
      29
    • Publisher
      廣川書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 薬学生のための細胞生物学2005

    • Author(s)
      堅田利明, 白土明子, 中西義信 他8名
    • Total Pages
      29
    • Publisher
      廣川書店
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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