• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Gene networks regulated by the HMG protein SOX2 in development

Research Project

Project/Area Number 16570173
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionOsaka University

Principal Investigator

KAMACHI Yusuke  Osaka University, Graduate School of Frontier Biosciences, Associate Professor, 大学院・生命機能研究科, 助教授 (90263334)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsSOX / N-cadherin / SOX2
Research Abstract

Group B1 Sox genes including Sox2 encode HMG domain transcription factors that play major roles in neural and placodal development, but little is known about gene regulatory networks involving the group B1 SOX proteins. In this project, we aimed to understand these networks.
Neural plate and sensory placodes share the expression of N-cadherin and Group B1 Sox genes, represented by Sox2. A 219-kb region of the chicken genome centered by the N-cadherin gene was scanned for neural and placodal enhancers. Random subfragments of 4.5 kb average length were prepared and inserted into tkEGFP reporter vector to construct a library with three-fold coverage of the region. Each clone was then transfected into N-cadherin-positive (lens, retina and forebrain) or -negative embryonic cells, or electroporated into early chicken embryos to examine enhancer activity. Enhancers 1 to 4 active in the CNS/placode derivatives, and non-specific enhancer 5 were identified by transfection, while electroporation of early embryos confirmed enhancers 2 to 4 as having activity in the early CNS and/or sensory placodes but with unique spatio-temporal specificities. Enhancers 2 to 4 are dependent on SOX binding sites, and mis-expression of Group B1 Sox genes in the head ectoderm caused ectopic development of placodes expressing N-cadherin, indicating the involvement of Group B1 Sox functions in N-cadherin regulation. Enhancers 1,2 and 4 correspond to sequence blocks conserved between the chicken and mammalian genomes, but enhancer 3 and 5 are unique to the chicken.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (19 results)

All 2006 2005 2004

All Journal Article (19 results)

  • [Journal Article] Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution.2006

    • Author(s)
      Okuda, Y. et al.
    • Journal Title

      Developmental Dynamics 235,3

      Pages: 811-825

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution.2006

    • Author(s)
      Okuda, Y., Yoda, H., Uchikawa, M., Furutani-Seiki, M., Takeda, H., Kondoh, H., Kamachi.Y.
    • Journal Title

      Dev.Dynam. 235(3)

      Pages: 811-825

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1.2006

    • Author(s)
      Takemoto, T., Uchikawa, M., Kamachi.Y., Kondoh, H.
    • Journal Title

      Development 133(2)

      Pages: 297-306

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolution.2006

    • Author(s)
      Okuda, Y.et al.
    • Journal Title

      Developmental Dynamics 235

      Pages: 811-825

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Multiple N-cadherin enhancers identified b systematic functional screening indicate its Group B1 SOX-dependent regulation in neural and placodal development.2005

    • Author(s)
      Matsumata, M et al.
    • Journal Title

      Developmental Biology 286,2

      Pages: 601-617

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1.2005

    • Author(s)
      Takemoto T. et al.
    • Journal Title

      Development 133,2

      Pages: 297-306

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional analysis of the chicken delta1-crystallin enhancer activity in Drosophila reveals remarkable evolutionary conservation between chicken and fly.2005

    • Author(s)
      Blanco, J. et al.
    • Journal Title

      Development 132,8

      Pages: 1895-1905

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Multiple N-cadherin enhancers identified by systematic functional screening indicate its Group B1 SOX-dependent regulation in neural and placodal development.2005

    • Author(s)
      Matsumata, M., Uchikawa, M., Kamachi, Y., Kondoh, H.
    • Journal Title

      Dev.Biol. 286(2)

      Pages: 601-617

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional analysis of the chicken delta1-crystallin enhancer activity in Drosophila reveals remarkable evolutionary conservation between chicken and fly.2005

    • Author(s)
      Blanco, J., Girard, F., Kamachi, Y., Kondoh, H., Gehring, WJ.
    • Journal Title

      Development 132(8)

      Pages: 1895-1905

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Multiple N-cadherin enhancers identified by systematic functional screening indicate its Group B1 SOX-dependent regulation in neural and placodal development.2005

    • Author(s)
      Matsumata, M et al.
    • Journal Title

      Developmental Biology 286

      Pages: 601-617

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1.2005

    • Author(s)
      Takemoto T.et al.
    • Journal Title

      Development 133

      Pages: 297-306

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Functional analysis of the chicken deltal-crystallin enhancer activity in Drosophila reveals remarkable evolutionary conservation between chicken and fly.2005

    • Author(s)
      Blanco, J.et al.
    • Journal Title

      Development 132

      Pages: 1895-1905

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells.2004

    • Author(s)
      Tanaka, S. et al.
    • Journal Title

      Molecular and Cellular Biology 24,20

      Pages: 8834-8846

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparison.2004

    • Author(s)
      Uchikawa, M. et al.
    • Journal Title

      Mechanism of Development 121,9

      Pages: 1145-1158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells.2004

    • Author(s)
      Tanaka, S., Kamachi, Y., Tanouchi, A., Hamada, H., Jing N., Kondoh, H.
    • Journal Title

      Mol.Cell.Biol. 24(20)

      Pages: 8834-8846

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparison.2004

    • Author(s)
      Uchikawa, M., Takemoto, T., Kamachi, Y., Kondoh, H.
    • Journal Title

      Mech.Dev. 121(9)

      Pages: 1145-1158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cells.2004

    • Author(s)
      Tanaka, S.et al.
    • Journal Title

      Mol.Cell.Biol. Vol.24 No.20

      Pages: 8834-8846

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparison.2004

    • Author(s)
      Uchikawa, M.et al.
    • Journal Title

      Mech.Dev. Vol.121 No.9

      Pages: 145-1158

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interplay of Pax6 and SOX2 in lens development as a paradigm of genetic switch mechanisms for cell differentiation.2004

    • Author(s)
      Kondoh et al.
    • Journal Title

      Int.J.Dev.Biol. Vol.48 No.8-9

      Pages: 819-827

    • Related Report
      2004 Annual Research Report

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi