Molecular mechanism enabling organ regeneration
| Project/Area Number |
16570180
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | University of Hyogo |
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Principal Investigator |
MOCHII Makoto University of Hyogo, Graduate School of Life Science, Associate Professor, 大学院生命理学研究科, 助教授 (90202358)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Xenopus / organ regeneration / gene expression / gene function / Xwnt-5a / XATF-5 |
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| Research Abstract |
To reveal a molecular mechanism underlying the organ regeneration, gene expression and functional analyses were performed as described below.
A comprehensive analysis using a cDNA array resulted in identification of up- or down-regulated genes during the tail regeneration of Xenopus laevis larva. The up-regulated genes were categorized into two groups, namely, the early responding and the late responding genes. Many genes in the late responding and down-regulated genes were expressed in a tissue-specific manner, suggesting their roles in cell differentiation. On the other hand, certain early genes were expressed in non-tissue-specific manner. Functions of some early genes were analyzed by mRNA injection into embryo. For example, Xenopus activating trascription factor 5 induced abnormality in neural tissues including brain and eye suggesting its role in cell growth in neural progenitor or stem cells.
Comparative study between a recovery process from a dorsal incision and the organ regeneration after the tail amputation revealed that a formation of a distal tip expressing Xwnt-5a was a specific event in the tail regeneration. To analyze the role of Xwnt-5a during the regeneration, an animal cap injected with Xwnt-5a mRNA was grafted into the incision. The result showed that the exogenous Xwnt-5a induced an ectopic tail in the recovering region and suggested that Xwnt-5a may be essential in the tail regeneration to pattern the distal to proximal axis.
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Report
(3 results)
Research Products
(18 results)
