| Project/Area Number |
16580005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Okayama University |
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Principal Investigator |
RIKIISHI Kazuhide Research Institute for Bioresources, Reseach Associate, 資源生物科学研究所, 助手 (90220798)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | wheat / pre-harvest sprouting / seed dormancy / mutant / ABA |
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| Research Abstract |
In this project, wheat mutants in reduced seed dormancy were analyzed for understanding the regulations of seed dormancy and pre-harvest sprouting in wheat.
1. ABA sensitivity in RSD (Reduced Seed Dormancy) mutants
RSD mutants showed lower sensitivity to exogenous ABA and the levels of sensitivity were different among mutant lines. Seed developmental stages affected ABA sensitivity of Norin 61 and RSD mutants and the different responses had shown among mutants.
2. Genetic analysis
Reduced seed dormancy of RSD14-1 and RSD12 were dominant mutations and those of RSD16-1 and RSD32 were recessive mutations. Segregation ratio for reduced seed dormancy in F2 population derived from the cross between Norin 61 and RSD32, suggests that mutant phenotype of RSD32 is a single recessive mutation.
3. Analysis of the regulation for a-amylase expression
RSD mutants showed the higher expression of a-amylase in embryos than Norin 61. Expression levels of genes that were negative regulator of a-amylase and show
… More
ed seed specific manner were determined. TaABF were expressed lower levels in three RSD mutants. Two RSD mutants also showed lower expression of TaAFP.
4. Regulation of ABA inducible genes in RSD mutants
Expression of Em genes that were induced by ABA and specifically expressed in seeds were lower level in RSD mutants, suggesting that ABA signals diminished in seed of RSD mutants. On the other hand, Norin 61 and RSD mutants showed no differences in the expressions of ABA inducible genes in seedlings. These results indicate that the mutations of RSD are specific in seed.
5. Comparison of gene expression by cDNA subtraction
Two clones (sbtc49 and sbtc63) were selected by the subtraction of cDNAs derived from embryos of Norin 61 and RSD32.
6. Proteomic analysis of two-dimensional protein-pattern
Quantitative and qualitative differences were detected in two-dimensional patterns of the protein extracted from embryos of Norin 61 and RSD32.
7. Screening of pre-harvest sprouting mutants
Twenty seven pre-harvest sprouting mutants (EPS: Enhanced Pre-harvest Sprouting) were screened by the germination in imbibed panicles from the population of mutagenized Norin 61 that showed resistance to pre-harvest sprouting. Less
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