Functional substance search of horticultural products and research of the action mechanism of a functional substance

• Project/Area Number: 16580028
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticulture/Landscape architecture
• Research Institution: University of Miyazaki
• Principal Investigator: ADACHI Masaru University of Miyazaki, 農学部, Associate professor (40353354)
• Co-Investigator(Kenkyū-buntansha): 武知 進土 (武知 進士) 崇城大学, 薬学部, 助教授 (10222100)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: horticultural crop / bioactive component / antioxidation / Sweetpotato / ポリフェノール / 抗酸化作用 / 抗ガン / アポトーシス / HL-60細胞 / キンカン / カンショ / Sweetpea / マンゴー

Research Abstract

Dihydropyrazine (DHP) species are included in the Maillard reaction intermediates. The Maillard reaction is thought to play a role in the pathophysiology of aging and diabetes. Thus, DHP may be involved in aging and diabetes related diseases, however, there is little reported the biological and physiological roles of DHPs. Moreover, polyphenolics(POPs) as a secondary metabolite in plant, have various physiological functions and play important roles in human nutrition. By using Escherichia coli mutant strains, we observed that cyclohexyl-DHP exposure strongly reduced the survival rate of a cytosolic sodium dodecyl sulfate (SOD)-deficient strain (sodA sodB), significantly reduced the survival rates of DNA repair-deficient strains (recA and uvrB) and mildly reduced the survival rate of a catalase-deficient strain (katE katG) compared with the survival rate of the wild-type strain. Addition of Cu(2+) enhanced the cell killing ability of cyclohexyl-DHP. The frequency of mutations induced by cyclohexyl-DHP increased dose-dependently in the sodA sodB strain. Moreover, cyclohexyl-DHP elevated the protein carbonyl levels in E caoli. We investigated the suppression of the proliferation of selected human cancer cells by phenolic compounds isolated from sweetpotato leaf. Caffeic acid and di-and tricaffeoylquinic acids dose-dependently depressed cancer cell proliferation, and the difference in sensitivity between caffeoylquinic acid derivatives and each kind of cancer cell was observed. Specifically, 3,4,5-tri-O-caffeoylquinic acid effectively depressed the growth of three kinds of cancer cells, and caffeic acid had an exceptionally higher effect against romyelocytic leukemia cells (HL-60) than other di- and tricaffeoylquinic acids.

Research Products

• [Journal Article] Oxidative stress induced by a dihydropyrazine derivative (2009)
  • Author(s): Takechi,S., Nakahara,K., Adachi,M., Yamaguchi,T.
  • Journal Title: Biol.Pharm.Bull. 32 Pages: 186-189
  • NAID: 110007042165
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Oxidative stress induced by a dihydropyrazine derivative (2009)
  • Author(s): Takechi, S., Nakahara, K., Adachi, M., Yamaguchi, T.
  • Journal Title: Biol.Pharm.Bull. 32 Pages: 186-189
  • NAID: 110007042165
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anti tri-ο-caffeoylquinic acid polyclonal antibody. I.Preparation and properties of polyclonal antibodies tocovalently coupled tri-ο-caffeoylquinic acid-albumin (2008)
  • Author(s): Kurata R., Adachi M., Kurata,H., Yoshimoto M.
  • Journal Title: Acta Horticulturae 770 Pages: 59-63
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Anti tri-o-caffeoylquinic acid polyclonal antibody. I. Preparation and properties of polyclonal antibodies to covalently coupled tri-o-caffeoylquinic acid-albumin (2008)
  • Author(s): Kurata R., Adachi M., Kurata, H., Yoshimoto M.
  • Journal Title: Acta Horticulturae 770 Pages: 59-63
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth suppression of human cancer cells by polyphenolics from sweetpotato(IPoRoea batatas L.)leaves (2007)
  • Author(s): Kurata R., Adachi M., Yamakawa O., Yoshimoto M.
  • Journal Title: J.Agr.Food Chem. 55・1 Pages: 185-190
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Growth suppression of human cancer cells by polyphenolics from sweetpotato(Ipomoea batatas L.)leaves (2007)
  • Author(s): Kurata R., Adachi M., Yamakawa O., Yoshimoto M.
  • Journal Title: J.Agr.Food Chem. 55 Pages: 185-190
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Growth Suppression of Human Cancer Cells by Polyphenilics from Sweetpotato (Ipomoea batatas L.) Leaves. (2007)
  • Author(s): Masaru ADACHI et al.
  • Journal Title: J. Agr. Food Chem. 55 Pages: 185-190
• [Journal Article] Anti tri-o-caffeoylquinic acid polyclonal antibody. I. Preparation and properties of polyclonal antibodies to covalently coupled tri-o-caffeoylquinic acid-albumin. (2007)
  • Author(s): Masaru ADACHI et al.
  • Journal Title: Acta Horticulturae 16(In press)
• [Journal Article] Mutation on spectrum induced by dihydropyrazines in Escherichia coli. (2006)
  • Author(s): Takechi,S., Yamaguchi,T., Nomura,H., Minematsu,T., Adachi,M., Kurata,H., Kurata,R.
  • Journal Title: Biol.Pharm. Bull. 29 Pages: 17-20
  • NAID: 110005602075
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mutation on spectrum induced by dihydropyrazines in Escherichia coli (2006)
  • Author(s): Takechi, S., Yamaguchi, T., Nomura, H., Minematsu, T., Adachi, M., Kurata, H., Kurata, R.
  • Journal Title: Biol.Pharm.Bull. 29 Pages: 17-20
  • NAID: 110005602075
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mutation spectrum by dihydropyrazines in Escherichia coli. (2006)
  • Author(s): Shinji TAKECHI et al.
  • Journal Title: Biol.Pharm.Bull. 29 Pages: 17-20
• [Journal Article] Radical species in DNA strand-cleavage caused by dihydropyr azines. (2005)
  • Author(s): Nobuhiro KASHIGE et al.
  • Journal Title: Biol.Pharm.Bull. 28 Pages: 419-423
• [Journal Article] The role of dihydropyrazines upon the addition of Copper(II) in an accelerated death of Escherichia coli. (2005)
  • Author(s): O TAKEDA et al.
  • Journal Title: Biol.Pharm.Bull. 28 Pages: 1161-1164
• [Journal Article] Mutation spectrum by dihydropyrazines in Escherichia coli. (2005)
  • Author(s): Shinji TAKECHI et al.
  • Journal Title: Mutation Research (in press)
• [Journal Article] Physiological active compound in petals of sweet pea(Lathyrusodoratus L.)flower (2004)
  • Author(s): Kurata,H., Adachi,M., Kurata R., Yoshimoto M., Nakamura,K., Hattanda,N., Nagata,R., Todoroki,A., Tatsumi,Y., Takechi,S.
  • Journal Title: Pol.Commun. 22 Pages: 443-444
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Induction of apoptotic cell death by polyphenolics from sweetpea(lathyrus odoratus L.)leaves in HL-60 cells (2004)
  • Author(s): Adachi,M., Kurata,H., Kurata R., Yoshimoto M., akamura,K., Hattanda,N., agata,R., Todoroki,A., Tatsumi,Y., Takechi,S.
  • Journal Title: Pol.Commun. 22 Pages: 441-442
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Physiological active compound in petals of sweet pea(Lathyrus odoratus L.)flower (2004)
  • Author(s): Kurata, H., Adachi, M., Kurata R., Yoshimoto M., Nakamura, K., Hattanda, N., Nagata, R., Todoroki, A., Tatsumi, Y., Takechi, S.
  • Journal Title: Pol.Commun. 22 Pages: 443-444
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induction of apoptotic cell death by polyphenolics from sweetpea(Lathyrus odoratus L.)leaves in HL-60 cells (2004)
  • Author(s): Adachi, M., Kurata, H, Kurata R., Yoshimoto M., Nakamura, K., Hattanda, N., Nagata, R., Todoroki, A., Tatsumi, Y., Takechi, S.
  • Journal Title: Pol.Commun. 22 Pages: 441-442
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Induction of apoptotic cell death by polyphenolics from sweetpea (Lathyrus odoratus L.) leaves in HL-60 cells. (2004)
  • Author(s): Masaru ADACHI et al.
  • Journal Title: Polyphenols Communications 2004 (22th International Conference on Polyphenols) 22 Pages: 441-442
• [Journal Article] Physiological active compound in petals of sweetpea (Lathyrus odoratus L.) flower. (2004)
  • Author(s): Hirofumi KURATA et al.
  • Journal Title: Polyphenols Communications 2004 (22th International Conference on Polyphenols) 22 Pages: 443-444
• [Journal Article] Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity. (2004)
  • Author(s): Shinji TAKECHI et al.
  • Journal Title: Mutation Research 560 Pages: 49-55
• [Journal Article] Familial amyotrophic lateral sclerosis with His46Arg mutation in Cu/Zn superoxide dismutase presenting characteristic clinical features and Lewy body-like hyaline inclusions. (2004)
  • Author(s): Shinji TAKECHI et al.
  • Journal Title: J.Neurol.,Sci. 225 Pages: 19-25