Establishment of general procedure to synthesize polysialogangliosides and its application for neurobidogy.
| Project/Area Number |
16580086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Gifu University |
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Principal Investigator |
ISHIDA Hideharu Gifu University, Faculty of Biological Science, Professor, 応用生物科学部, 教授 (20203002)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | ganglioside / sialic acid / ceramide / sphingo-glycolipid |
|---|
| Research Abstract |
Glycosphingolipids are characteristic membrane components of eukaryotic cells where they are distinguished from other glycosphingolipids by containing one or more sialic acid residues, and have been recognized play important roles in many biological processes, particularly in nervous system We have succeeded in the establishment of general procedunes to synthesize polysialogangliosides. The achievements are summarized in the following two parts.
1.Efficient construction of polysialo-oligosaccharides.
α(2-4)- and α(2-8)disialic acid seguences were obtained in high yields by employing 1,5-lactamized sialyl acceptors. The disaccharides obtained were utilized for the construction of sialo-oligosaccharides.
2.The coupling of oligosaccharides and ceramides by intramolecular-aglycon derivery.
Tethering of ceramide to glucose, reducing terminal of oligosaccharides, with succnic acid allowed the efficient coupling of oligosaccharides and ceramides. The glucosyl ceramide obtained was further glycosylated with sialo-oligosaccharides as building bloks to give the desired gangliosides.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Studies on the α-(1→4)-and α-(1→8)-fucosylation of sialic acid for the total assembly of the glycan portions of complex HPG-series gangliosides2006
Author(s)
Ando, H., Shimizu, H., Katano, Y., Koike, Y., Koizumi, S., Ishida, H., Kiso, M.
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Journal Title
Carbohydrate Research 341
Pages: 1522-1532
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Ando T. : Synthesis and enzymatic susceptibility of a series of novel GM2 analogs2006
Author(s)
Fuse, T., Ando, H., Imamura, A., Sawada, N., Ishida H., kiso, M.
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Journal Title
Glycoconjugate Journal 23
Pages: 329-343
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] 1,5-Lactamized sialyl acceptors for various disialoside syntheses : Novel synthesis method for glycan portions of Hp-s6 and HLG-2 gangliosides2005
Author(s)
Ando, H., Koike, Y., Koizumi, S., Ishida, H., Kiso, M.
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Journal Title
Angewandte Chemie, International Edition in English
Pages: 6759-6763
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Surface plasmon resonance study of carbohydrate-carbohydrate interaction between various gangliosides and Gg3-carring polystyrene2004
Author(s)
Matsuura, K., Oda, R., Kitakouji, H., Kiso, M., Kitajima, K., Kobayashi, K.
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Journal Title
Biomacromolecules 5
Pages: 937-941
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[Journal Article] Loss of disialyl Lewis^a, the ligand for lymphocyte inhibitor receptor sialic acid-binding immunoglobulin-like lectin-7 (siglec-7) associated with increased sialyl Lewis^a expression on human colon cancers2004
Author(s)
Miyazaki, K., Ohmori.K., Izawa, M., Koike, T., Kumamoto, K., Furukawa, K., Ando, T., Kiso, M., Yamaji, T., Hashimoto.Y., Suzuki, A., Yoshida, A., Takeuchi, M, Kannagi, R
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Journal Title
Cancer Res. 64
Pages: 4498-4505
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[Book] 未来を拓く 糖鎖化学2005
Author(s)
石田 秀治
Total Pages
467
Publisher
(株)金芳堂
Description
「研究成果報告書概要(和文)」より
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[Book] 糖鎖科学の新展開2005
Author(s)
安藤 弘宗
Total Pages
536
Publisher
(株)エヌ・ティ・エス
Description
「研究成果報告書概要(和文)」より
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