Apoptosis-inducing activity of plant lectins and specific application to tumor cells
| Project/Area Number |
16580091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | University of Shizuoka |
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Principal Investigator |
ISEMURA Mamoru University of Shizuoka, School of Food and Nutritional Sciences, Professor, 食品栄養科学部, 教授 (40028197)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | lectin / cytokine / anti-tumor / apoptosis / udo / kurokawa / wasabi / mistletoe / 免疫機能 / 癌細胞 / ブタ血漿 / インターロイキン / 遺伝子発現 |
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| Research Abstract |
Lectins are proteins of non-immune origin with specific binding affinity for carbohydrate moiety of glycoconjugates. We have developed a new method to isolate lectins using affinity gels conjugated with porcine plasma proteins. In this study, we examined biological activities of lectins isolated by our method. Aralia cordate lectin (ACL) from udo showed activity to enhance gene and protein expression of proinflammatory cytokine IL-8 in Caco-2 cells. Our results also showed apoptosis inducing activity of Japanese mistletoe lectin, iris lectin, and muscari lectin in human leukemia U937 cells. In addition, we found that Boletopsis leucomelas lectin (BLL) from the edible mushroom kurokawa induced apoptosis in U937 cells by the mechanism incuding the G2/M cell cycle arrest in assocation with up-regulation of p21/Waf1 gene expression. The observed decrease in expression of bcl-2 was ascribable to the up-regulated expression of p21/Waf1 and correlated to the enhanced release of cytochrome c from mitochondria to cytoplasm, leading to the activation of caspase-9 in BLL-treated cells. These data suggest usefulness of our new method for lectin isolation and importance of examining biological activities of plant lectins in various animal systems.
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Report
(3 results)
Research Products
(14 results)
