Studies on the promoting effects of cumulus cells on cytoplasmic maturation of bovine immature oocytes during in vitro maturation culture.
| Project/Area Number |
16580228
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
YAMADA Masayasu Kyoto Univ., Grad.School of Agri., Associate Professor, 農学研究科, 助教授 (10243073)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | bovine / CEOs / midkine / retinoic acid / carnitine / cytoplasmic maturation / apoptosis / IVM / ウシ未成熟卵母細胞 / 卵丘細胞 / アンチセンスオリゴヌクレオチド |
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| Research Abstract |
The competence of postfertilization development to blastocysts of bovine oocytes is acquired during in vitro maturation (IVM) period. The developmental competence, referred to as cytoplasmic maturation, is promoted by midkine (MK). MK is a retinoic acid (RA)-inducible and heparin binding growth factor. Recently it was found that apoptosis occurred in cumulus cells of bovine cumulus-enclosed oocytes (CEOs) during IVM, suggesting that the degree of apoptosis in cumulus cells of CEOs may be correlated with their cytoplasmic maturation. The present study was undertaken to examine the effects of MK and RA on cytoplasmic maturation of bovine CEOs and apoptosis of their cumulus cells during IVM period. As a result, it was found that MK and RA had cytoplasmic maturation-promoting activities. Ligation-madiated PCR experiments revealed that the degree of apoptotic internucleosomal DNA fragmentation in cumulus cells of CEOs was significantly lower in MK- or RA-treated CEOs compared to the untreated control group. Next, the effects of carnitine, which has an anti-apoptotic activity to various types of cells, on cytoplasmic maturation of bovine CEOs were examined, because carnitine transporter was detected both in oocytes and cumulus cells of CEOs using immunofluorescence method. It was found that transition of active mitochondria from cortex region into inner cytoplasm as well as cytoplasmic maturation of CEOs were promoted by carnitine treatment during IVM. Finally, suppression subtractive hybridization analysis revealed that expression of some genes such as topoisomerase α, apoptosis related gene and ovary specific gene OVN6-2 in cumulus cells were promoted by MK treatment.
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Report
(3 results)
Research Products
(9 results)
