Brain development and evolution from the aspect of cerebrovascular reactivity and drug distribution of receptor subtypes

• Project/Area Number: 16580242
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Kagoshima University
• Principal Investigator: MIYAMOTO Atsushi Kagoshima University, Agriculture, Professor, 農学部, 教授 (70219806)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000); Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: cerebral artery / vasoreactivity / receptor / fish / amphibia / reptile / fowl / mammal

Research Abstract

1. Adrenoceptor a and 5-HTi receptor subtypes might be important in arterial contraction in snake basilar artery. Endothelial cells might play an important role in the responsiveness of snake basilar arteries to rattlesnake BK, but they might not be involved in the responsiveness to ACh, BK and resting vascular tone.
2. NO may inhibit COX or TXA_2 synthetase, and that therefore inhibition of NOS might disinhibit COX or TXA_2 synthetase, subsequently inducing TXA_2 production in porcine basilar arterial endothelial cells. On the other hand, as TXA_2 and other contractility-related prostaglandin(s) may inhibit NOS, therefore the inhibition of COX or TXA_2 synthetase might disinhibit NOS, and then increase the spontaneous production of NO.
3. The contraction induced by Ang II might be mediated via the activation of ATi receptors on the porcine basilar arterial smooth muscle cells and be modulated via the activation of AT_2 receptors on the endothelial cells, followed by NO production.
4. Endotoxin enhances NO production in Mg-deficient rat aortas directly, and that endotoxin receptors might, at least in part, contribute to this enhancement.

Research Products

• [Journal Article] Vasomotor Effects of Noradrenaline, Acetylcholine, Histamine, 5-Hydroxytriptamine and Bradykinin on Snake (Trimeresurus flavoviridis) Basilar Arteries. (2007)
  • Author(s): Yoshinaga, N., Okuno, T., Watanabe, Y., Matsumoto, T., Shiraishi, M., Obi, T., Yabuki, A., Miyamoto, A.
  • Journal Title: Comp. Biochem. Physiol. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Ibuprofen or ozagrel increses NO release and L-nitro arginine induces TXA_2 release from cultured porcine basilar arterial endothelial cells. (2007)
  • Author(s): Miyamoto, A., Hashiguchi, Y., Obi, T., Ishiguro, S., Nishio, A.
  • Journal Title: Vascul. Pharmacol. 46・2 Pages: 85-90
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Vasomotor Effects of Noradrenaline, Acetylcholine, Histamine, 5-Hydroxytriptamine and Bradykinin on Snake (Trimeresurus flavoviridis) Basilar Arteries (2007)
  • Author(s): Yoshinaga, N, Okuno, T., Watanabe, Y., Matsumoto, T., Shiraishi, M., Obi, T., Yabuki, A., Miyamoto, A.
  • Journal Title: Comparative Biochemistry and Physiology ( C) (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ibuprofen or ozagrel increses NO release and L-nitro arginine induces TXA2 release from cultured porcine basilar arterial endothelial cells. (2007)
  • Author(s): Miyamoto, A., Hashiguchi, Y., Obi, T., Ishiguro, S., Nishio, A.
  • Journal Title: Vascular Pharmacology 46(2) Pages: 85-90
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ibuprofen or ozagrel increses NO release and L-nitro arginine induces TXA_2 release from cultured porcine basilar arterial endothelial cells. (2007)
  • Author(s): Miyamoto, A., et al.
  • Journal Title: Vascular Pharmacology 46・2 Pages: 85-90
• [Journal Article] Role of angiotensin II receptor subtypes in porcine basilar artery : Functional, radioligand binding, and cell culture studies. (2006)
  • Author(s): Miyamoto, A., Wada, R., Inoue, A., Ishiguro, S., Liao, J.K., Nishio, A.
  • Journal Title: Life Sci. 78 Pages: 943-949
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Role of angiotensin II receptor subtypes in porcine basilar artery : Functional, radioligand binding, and cell culture studies. (2006)
  • Author(s): Miyamoto, A., Wada, R., Inoue, A., Ishiguro, S., Liao, J.K., Nishio, A.
  • Journal Title: Life Sciences 78(9) Pages: 155-162
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of angiotensin II receptor subtypes in porcine basilar artery : functional, radioligand binding, and cell culture studies. (2006)
  • Author(s): Miyamoto, A., et al.
  • Journal Title: Life Sciences 78・9 Pages: 155-162
• [Journal Article] Role of angiotensin II receptor subtypes in porcine basilar artery : functional, radioligand binding, and cell culture studies. (2006)
  • Author(s): Miyamoto, A.
  • Journal Title: Life Sci. 78(9) Pages: 155-162
• [Journal Article] In vitro application of endotoxin enhances nitric oxide production in thoracic aorta from Mg-deficient rats. (2005)
  • Author(s): Miyamoto, A., Moriki, H., Ishiguro, S., Nishio, A.
  • Journal Title: Magnesium Res. 18 Pages: 155-162
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In vitro application of endotoxin to thoracic aortas from magnesium-deficeint rats enhances vascular hyporeactivity to phenylephrine. (2004)
  • Author(s): Miyamoto, A., Moriki, H., Ishiguro, S., Nishio, A.
  • Journal Title: J. Am. Coll. Nutr. 23
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] In vitro application of endotoxin to thoracic aortas from magnesium-deficeint rats enhances vascular hyporeactivity to phenylephrine. (2004)
  • Author(s): Miyamoto, A., Moriki, H., Ishiguro, S., Nishio, A.
  • Journal Title: J. Am. Coll. Nutr 23
  • Description: 「研究成果報告書概要(欧文)」より