| Project/Area Number |
16580261
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Iwate University |
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Principal Investigator |
SATO Reeko IWATE UNIVERSITY, AGRICULTURE, PROFESSOR, 農学部, 教授 (80142892)
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| Co-Investigator(Kenkyū-buntansha) |
NAITO Yoshihisa IWATE UNIVERSITY, AGRICULTURE, PROFESSOR, 農学部, 教授 (40003785)
YASUDA Jun IWATE UNIVERSITY, AGRICULTURE, PROFESSOR, 農学部, 教授 (20142705)
INANAMI Osamu HOKKAIDO UNIVERSITY, GRADUATE SCHOOL OF VETERINARY MEDICINE, ASSOCIATE PROFESSOR, 大学院・獣医学研究科, 助教授 (10193559)
寺口 進 森永乳業栄養科学研究所, 基礎科学研究室, 室長
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | lactoferrin / feline immunodeficiency virus (FIV) / immunodeficiency / chronic inflammation / cat / lymphocyte proliferation / interferon-γ / cytokine / PBMC / IFN-γ |
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| Research Abstract |
1)The effects of orally-administered bovine lactoferrin (bLF) on lymphocyte functions in FIV-infected cats
The effects of bLF on ConA-induced proliferation of peripheral lymphocyte in healthy and FIV-infected cats were investigated. Orally-administered bLF showed the inhibition of lymphocyte proliferation both in healthy and FIV- infected cats. Co-incubation of bLF and peripheral lymphocytes also showed the inhibition of lymphocyte proliferation both in healthy and FIV-infected cats.
2)The effects of bLF and ConA on cytokine mRNA expression in feline peripheral lymphocyte
After the co-incubation of ConA and peripheral lymphocyte, mRNA expression of IFN-γ,IL-1β,TNF-α and IL-12p40 was investigated by RT-PCR method. The strong mRNA-expression of IFN-γ was first detected at 4hr after the addition of ConA, however the addition of ConA did not bring the mRNA-expression of other cytokines such as IL-1(3,TNF-α and IL-12p40. After that, the mRNA-expression of IFN-γ was investigated by quantitative
… More
analysis with real time PCR in order to confirm the result of RT-PCR. We established the method of real time PCR for feline IFN-γ. After the stimulation of ConA, the strong mRNA-expression of IFN-γ was observed in peripheral lymphocyte both of healthy and FIV-infected cats, using real time PCR method. Before ConA stimulation and 10, 20 and 40 min after ConA stimulation, bLF was added to peripheral lymphocyte and ConA-induced mRNA- expression of IFN-γ was determined by real time PCR. Bovine LF inhibited the ConA-induced mRNA-expression of IFN-γ in felime lymphocyte. The inhibition of IFN-γ-mRNA-expression was observed not only when lymphocyte was treated with bLF 30 min before but also 10,20 or 40 min after ConA stimulation.
3)The effects of Con A stimulation on the lymphocyte signaling pathways of IFN-γ-mRNA-expression
The relation of ConA stimulation and signaling pathways of lymphocyte was investigated. Our results suggested that PTK-and ERK-dependent signaling pathways are involved in the ConA-induced expression of IFN-γ-mRNA. Therefore, it is likely that bLF inhibits IFN-γ production by interfering with this signaling pathway.
Judging from these results, the beneficial effects of bLF in vivo may be explained by modulatory ability of bLF against feline lymphocyte. These modulatory actions of bLF seemed to be involved in anti-inflammatory effects on the cats with chronic inflammation. Less
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