Application of autotransfusion with -80℃ canine erythrocyte cryopreservation in veterinary medicine

• Project/Area Number: 16580262
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Yamaguchi University
• Principal Investigator: TAURA Yasuho Yamaguchi University, Faculty of Agriculture, Professor, 農学部, 教授 (80163153)
• Co-Investigator(Kenkyū-buntansha): NAKAICHI Munenazu Yamaguchi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60243630) ; UNE Satoshi Yamaguchi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60294659)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000)
• Keywords: canine / -80℃ / autotransfusion / hydroxyethyl starch (HES) / erythrocyte / cryopreservation / veterinary medicine / cryoprotectant

Research Abstract

In this canine erythrocytes study, the effect of -196℃ or two step method(-196℃→80℃) cryopreservation was examined. Erythrocytes were mixed with cryoprotective solution containing hydroxyethyl starch (HES) or glycerol (GL). This mixture was filled into plastic bag made of vinyl chloride and frozen to -196℃ in liquid nitrogen. It was transferred to -80℃ deepfreezer and was preserved for 3 month. After thawing, erythrocytes were not washed, and autotransfused.
1)The canine erythrocytes frozen HES and GL at -196℃ could be autotransfused without side effects. The HES was considered to have a practical value rather than the GL.
2)HES was used a cryoprotective solution, the possibility of the plentiful cryopreservation of canine erythrocytes at two step method (-196℃→-80℃) was found out.
3)Canine erythrocytes were cryopreserved at -80℃ with HES for three months or more was found out. In recovery rate, osmotic fragility test and scanning electron microscopy, significant difference was not observed in each period. Therefore, -80℃ cryopreservation can preserve canine erythrocytes longer than liquefied preservation.
4)The canine erythrocytes frozen HES at two step method could be autotransfused without side effects.
This two step method that could be autotransfused, and was considered to have a practical value in veterinary medicine.

Research Products

• [Journal Article] Incubation of canine red blood cells before freezing leads to an improuved saline stability and morphology after thawing when using a hydroxyethyl starch cryopresevation protocol. (2005)
  • Author(s): Kim, H, et al.
  • Journal Title: Low Temp.Med 31 Pages: 33-35
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch. (2005)
  • Author(s): Kim, H, et al.
  • Journal Title: Cryo Letters 26・1 Pages: 1-6
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Incubation of canine red blood cells before freezing leads to an improuved saline stability and morphology after thawing when using a hydroxyethyl starch cryopresevation protocol. (2005)
  • Author(s): Kim, H., Itamoto, K., Tanaka, S., Nakaichi, M., Sumida, S., Taura, Y.
  • Journal Title: Low Temperature Medicine Vol.31 Pages: 33-35
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch. (2005)
  • Author(s): Kim, H., Itamoto, K., Une S., Nakaichi, M., Taura, Y., Sumida, S.
  • Journal Title: Cryo Letters. Vol.26 Pages: 1-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Incubation of canine red blood cell before freezing leads to an improved saline stability and morphology after thawing when using a hydroxyethyl starch cryopreservation protocol. (2005)
  • Author(s): Kim, H., Itamoto, K., Tanaka, S., Nakaichi, M., Sumida, S., Taura, Y.
  • Journal Title: Low Temp.Med. 31 Pages: 33-35
• [Journal Article] Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch. (2005)
  • Author(s): Kim, H., Itamoto, K., Une, S., Nakaichi, M., Taura, Y., Sumida, S.
  • Journal Title: Cryo Letters 26.1 Pages: 1-6
• [Journal Article] A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation.Dec;(2004) (2004)
  • Author(s): Kim, H.et al.
  • Journal Title: J Vet Med Sci 66・12 Pages: 1543-1547
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation. (2004)
  • Author(s): Kim, H., Tanaka, S., Une, S., Nakaichi, M., Sumida, S., Taura, Y.
  • Journal Title: Journal Veterinary Medical Science Vol.66 Pages: 1543-1547
  • NAID: 10014192453
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopredervation. (2004)
  • Author(s): Kim, H., Tanaka, S., Une, S., Nakaichi, M., Taura, Y.
  • Journal Title: J.Vet.Med.Sci 66.12 Pages: 1543-1547