| Project/Area Number |
16590094
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Teikyo University |
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Principal Investigator |
KAWASHIMA Kohtaro Teikyo University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (20124993)
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| Co-Investigator(Kenkyū-buntansha) |
NEGISHI Yoichi Tokyo University of Pharmacy and Life Science, School of Pharmacy, Lecturer, 薬学部, 講師 (50286978)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | exposure of hyper-gravity / quasi-microgravity / osteoclast / RAW276.4 cells / clinostat / parathyroid hormone / 1α,25(OH)_2D_3 / prostaglandin |
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| Research Abstract |
Bone destruction proceeds in the space. It is reported that this phenomenon depends on the decrease of the osteoblastic activity. But it is not known whether the osteoclastic activity is important or not for the bone loss in the space.
This study focused on the formation and the activation of osteoclasts induced by the exposure of the hypergravity or microgravity. Hypergravity and quasi-microgravity were obtained by the centrifugation and by the clinostat culture, respectively.
The formation of osteoclasts were observed in the following two methods, the first is the mouse bone marrow culture and the second is the culture of RAW276.4 cells in which the osteoclasts are induced by the RANKL.
Both hypergravity and quasi-microgravity had no effects on the formation of osteoclasts induced by active vitaminD_3, parathyroid hormone, prostaglandins in the mouse bone marrow culture. The induction of osteoclasts from RAW276.4 cells were also not influenced by both hypergravity and quasi-microgravity. Hypergravity had no effects on the bone-resorbing activity of rat fetal bone. Therefore, no changes were observed in the formation and activation of osteoclasts by the gravitational alteration.
Exposure of hypergravity is known to increase the function of osteoblasts and, on the contrary, microgravity in the space is known to decrease. We first speculated that the osteoclasts also have the important role on the bone destruction as well as the osteoblasts in the space. But, our results shows that decreased function of osteoblasts are important for the bone loss in the space and the osteoclasts has the minor role.
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