Research Project
Grant-in-Aid for Scientific Research (C)
HA-c-Cbl was expressed in HEK293 cells and immunoprecipitated. Insulin receptor expressed in HIRc cells was immunoprecipitaed. Then Both immunoprecipitants were mixed with E1,UbcH7 as an E2,ATP and its regenerating system, ubiquitin or its mutants. These mixtures were reacted in pH6.0, 6.5 and 7.2. Ubiquitinated insulin receptor were detected by immunoblot. When the reaction was done at pH7.2, insulin receptor were monoubiquitinated with multiple lysine residues in insulin receptor, whereas when the reaction was carried out at pH6.0, the insulin receptor was subjected to polyubiqutination through Lys-48 in ubiquitin molecules. Therefore, I projected next to analyze the structural change of recombinant c-Cbl between neutral pH and acidic pH.Next, I was planed to analyze the ubiquitination profiles of epidermal growth factor receptor and platelet derived growth factor receptor. These projects would be under going.Recently, I identified the monoubiquitination of LDH-A, a cytosolic protein, under oxidative stress was sequestered into and degraded in lysosomes. Now, I would search the ubiquitin receptor on the cytosolic surface of lysosomal membranes.
All 2005 2004 Other
All Journal Article (33 results) Book (2 results)
Br J Dermatol 153・1
Pages: 37-45
Biochem Biophys Res Commun 336・3
Pages: 799-806
Int J Parasitol 25・14
Pages: 1587-1594
J Bone Mineral Res (In press)
Curr.Eye Res. 28
Pages: 373-379
Br.J.Dermatol. 153
Biochem.Biophys.Res.Commun. 336
Int.J.Parasitol. 25
Int J Parasitol. 35・14
Biochem.Biophys.Res.Commun. 336・3
Br.J.Dermatol. 153・1
FASEB J 18・3
Pages: 522-524
Pathol Int 54・5
Pages: 322-331
J Cell Sci 117・4
Pages: 2997-3009
J Biol Chem 279・33
Pages: 34827-34832
Biol Chem 385・6
Pages: 511-515
J Dermatol Sci 36・1
Pages: 60-62
Curr Eye Res. 28・6
内分泌・糖尿病化 18・4
Pages: 352-357
FASEB.J. 18
Pathology Int. 54
J.Cell Sci. 117
J.Dermatol Sci. 36
Biol.Chem. 385
Pages: 511-516
J.Biol.Chem. 279
Curr.Eye Research 28
J Biol Chem 279
Biol Chem 385
J Cell Sci 117
FASEB J 18
J.Bone Mineral Res. (In press)
J Bone Meneral Res (In press)
Br.J.Dermatol in press