Identification of Tumor Antigen and Analysis of the Responses for the Tumor Antigen
| Project/Area Number |
16590315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Okayama University |
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Principal Investigator |
UENAKA Akiko Okayama University, Graduate School of Medicine, Dentistry and Pharmacology, Lecturer, 大学院・医歯薬学総合研究科, 講師 (50273967)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | tumor / antigen / ELISPOT cloning / IFNγ / monoclonal antibody / NY-ESO-1 / monitoring / 細胞・組織 / 腫瘍抗原 / 細胞傷害性T細胞(CTL) / エリスポトクローニング / 腎癌 / 肺癌 / Meth A / RL male 1 |
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| Research Abstract |
CD8 T cells can recognize MHC and peptide on the cell surface and kill the cells directly. Increasing the effector CD8 T cells recognizing tumor by vaccination of tumor rejection antigen or epitope peptides is an expectation for therapies of cancer. The identification of the tumor rejection antigens and CTL epitope peptides is a main theme for this purpose.
Recently, we improved the new method of T cell antigen cloning with the sensitivity and the efficacy by using large and small-scale ELISPOT assays. In mouse model system, the tumor rejection antigen of Meth A sarcoma, ramp (retinoic acid-regulated nuclear matrix-associated protein) was identified by ELISPOT-cloning by using this new improved method. For identification of human-lung-cell-carcinoma antigen recognized by tumor specific CD8 T cells, the cDNA library was prepared from mRNA of tumor cells and transfected them into 293T cells with HLA gene. And then assayed the transfected 293T cells to stimulate autologous tumor specific CD8 T cells to produce IFNγ. We have finished the first and second screening by large-scale ELISPOT assay.
For analysis of the expression and cellular localization of the tumor antigen, XAGE-1b, defined by SEREX, mAbs for XAGE-1b were produced.
On going phase I study of the CHP-NY-EO-1 vaccination, NY-EO-1 specific CD4-T and CD8-T cell responses were analyzed pre and after vaccination. The increased responses of CD4 T cells were found after the second vaccination by IENγ secretion assay.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] In vitro stimulation of CD8 and CD4 T cells by dendritic cells loaded with a complex of cholesterol-bearing hydrophobized pullulan and NY-ESO-1 protein : Identification of anew HLA-DR15-binding CD4 T-cell epitope.2006
Author(s)
Hasegawa K., Noguchi Y., Koizumi F., Uenaka A, Tanaka M, Shimono M., Nakamura H., Shiku H., Gnjatic S., Murphy R., Hiramatsu Y., Old LJ., Nakayama E.
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Journal Title
Clin Cancer Res 12(6)
Pages: 1921-1927
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] XAGE-1 expression in non-small cell lung cancer and antibody response in patients.2005
Author(s)
Nakagawa K., Noguchi Y., Uenaka A., Sato S., Okumura H., Tanaka M., Shimono M., Eldib AMA., Ono T., Ohara N., Yoshino T., Yamashita K., Tsunoda T., Aoe M., Shimizu N., Nakayama E.
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Journal Title
Clin Cancer Res 11(15)
Pages: 5496-5503
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Identification of glioma-specific RFX4-E and -F isoforms and humoral immune response in patients.2005
Author(s)
Matsusita H., Uenaka A., Ono T., hasegawa K., Sato S., Koizumi F., Nakagawa K., Toda M., shingo T., Ichikawa T., Noguchi Y., Tamiya T., Furuta T., Kawase T., Date I., Nakayama E.
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Journal Title
Cancer Sci 96(11)
Pages: 801-809
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Book] 免疫学的プロトコール2004
Author(s)
田中志幸, 上中明子, 中山睿一, 中内啓光編
Total Pages
243
Publisher
羊土社
Description
「研究成果報告書概要(和文)」より
Related Report
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