Elucidation of molecular basis and activation mechanism for gastrointestinal Nox1 by RNA interference
| Project/Area Number |
16590598
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KONDO Shigetada The University of Tokushima, Institute of Health Bioscience, Assistant professor, 大学院・ヘルスバイオサイエンス研究部, 助手 (40304513)
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| Co-Investigator(Kenkyū-buntansha) |
ROKUTAN Kazuhito The University of Tokushima, Institute of Health Bioscience, Professor, 大学院・ヘルスバイオサイエンス研究部, 教授 (10230898)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | large intestinal epithelial cells / innate immunity / reactive oxygen species / superoxide anion / Nox1 / gastric epithelial cells / Nox1 oxidase |
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| Research Abstract |
NADPH oxidase 1 (Nox1), a homolog of phagocyte gp91-phox, is dominantly expressed in gastric and large intestinal epithelium, and reactive oxygen species derived from Nox1 are suggested to serve a role in host defense against bacteria in gastrointestinal tract.
We elucidated that
1.primary cultures of guinea pig gastric and large intestinal epithelial cells expressed the Nox1, p22-phox, p67-phox, Nox organizer (Noxo1), Nox activator (Noxa1), and Rac1 mRNAs and proteins.
2.Treatment of human colon cancer T84 cells with recombinant flagellin from Salmonella enteritidis augmented the superoxide anion release in association with the induction of Nox1 protein.
3.Helicobacter pylori lipopolysaccharide activates Rac1 and transcription of Nox1 and Noxo1 in guinea pig gastric mucosal cells.
4.interferon-gamma, a crucial inducer of the gp91-phox gene, also stimulates expression of Nox1 through an activation of the promoter for nox1 gene.
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Report
(3 results)
Research Products
(21 results)
