| Project/Area Number |
16590706
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TATSUMI Tetsuya Kyoto Prefectural University of Medicine, Assistant Professor, 医学研究科, 講師 (20254328)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUBARA Hiroaki Kyoto Prefectural University of Medicine, Professor, 医学研究科, 教授 (10239072)
KOBARA Miyuki Kyoto Pharmaceutical University, Associate Professor, 薬学部, 助教授 (80275198)
KEIRA Natsuya Kyoto Prefectural University of Medicine, Assistant, 医学研究科, 助手 (50267829)
白石 淳 京都府立医科大学, 医学研究科, 助手 (10372848)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | angiogenesis / acute myocardial infarction / bone marrow implantation / peripheral blood-derived mononuclear cells / erythropoietin / cardiac function |
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| Research Abstract |
Background : We have reported that transplantation of non-expanded peripheral blood-derived mononuclear cells (PBMNCs) enhances neovessel formation in ischemic myocardium and limbs by releasing angiogenic factors. This study was designed to examine whether intracoronary transplantation of PBMNCs improves cardiac function after acute myocardial infarction (AMI). Methods : After successful percutaneous coronary intervention (PCI) for a ST-elevation AMI with occlusion of proximal left anterior descending coronary artery (LAD) within 24 hours, patients were assigned to either control (PCI alone) group or PBMNC group that received intracoronary infusion of PBMNCs within 5 days after PCI. We obtained PBMNCs (4.92±2.82x10^9 cells) from patients by use of COBE spectra apheresis with mononuclear cell collection methods and concentrated to 10 ml, 3.3 ml of which was infused via over-the-wire catheter under 3 min low pressure balloon inflation (3 times). Primary endpoint was global left ventricul
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ar ejection fraction (LVEF) change from baseline to 6 months' follow-up. Results : Our data show that the absolute increase in LVEF (AEF) was 7.4% in control group and 13.4% (p=0.037 vs control) in PBMNC group. Cell therapy resulted in a greater tendency of ΔRegional EF or significant improvement of wall motion score index (WMSI) and Tc-99m-tetrofosmin perfusion defect score associated with the infarct area, compared with control. Moreover, intracoronary administration of PBMNCs did not exacerbate left ventricular (LV) end-diastolic and end-systolic volume expansion or high risk arrhythmia without any adverse clinical events. Conclusion : Intracoronary infusion of non-expanded PBMNCs promotes improvement of LV systolic function. This less invasive and more feasible approach to collect EPCs may provide a novel therapeutic option to improve cardiac function against AMI. We will plan to examine the efficacy of this novel angiogenic cell therapy against AMI in combination with erythropoietin administration. Less
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