| Project/Area Number |
16590897
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | KURUME UNIVERSITY |
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Principal Investigator |
YAMADA Kentaro Kurume University, School of Medicine, Professor, 医学部, 教授 (10191305)
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| Co-Investigator(Kenkyū-buntansha) |
OTABE Shuichi Kurume University, School of Medicine, Assistant professor, 医学部, 助手 (70194553)
TAKANE Naoko Kurume University, School of Medicine, Lecturer, 医学部, 講師 (70261071)
NAKAYAMA Hitomi Kurume University, School of Medicine, Assistant professor, 医学部, 助手 (20368955)
TAKATA Kazuna Kurume University, School of Medicine, Assistant professor, 医学部, 助手 (30368956)
KURITA Yayoi Kurume University, School of Medicine, Assistant professor, 医学部, 助手 (40368957)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | adiponectin / transgenic mouse / metabolic syndrome / obesity / hyperlipidemia / KK-A^y mouse / トランスジェニックマウス / 代謝症候群 |
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| Research Abstract |
To assess the effects of chronic hyperadiponectinemia on metabolic phenotypes, we established 3 lines of Transgenic mice expressing human full length adiponectin on B6 background. On high fat/high sucrose diet, the transgenic mice expressing high levels of adiponectin exhibited significantly decreased weight gain associated with less fat accumulation and smaller adipocytes in both the visceral and subcutaneous adipose tissues than wild-type B6 mice. The transgenic mice had higher rectal temperature, and greater daily oxygen consumption with a lower respiratory quotient. Moreover, high-calorie diet-induced oxidative DNA damage was significantly attenuated in the hyperadiponectinemic mice, despite unaltered food consumption. The human adiponectin transgene was transferred to KK-A^y mice by repeated back crosses. KK-A^y mice expressing human adiponectin also showed on regular chow elevated oxygen consumption rate, reduced weight gain, and improved insulin sensitivity to exogenous insulin, confirming the beneficial effects of adiponectin on obesity-related metabolic disorders.
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