| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Satoshi The University of Tokyo, Institute of Medical Science, Associate Professor, 医科学研究所, 助教授 (60226834)
TOJO Arinobu The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (00211681)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
Human herpesvirus 6 (HHV-6) is classified into variants A and B. HHV-6B cause significant complications after hematopoietic stem cell transplantation (SCT) including encephalitis, pneumonia, and bone marrow suppression. We studied HHV-6 infection in 23 adults after cord blood transplantation (CBT) using a real-time quantitative PCR method on serum samples. In 20 of 23 CBT patients (87%), HHV-6B DNA was found in at least one serum sample. HHV-6B DNAemia was found in 15 patients (65%) at week 2, 16 (70%) at week 3, and three (13%) at week 4. However, HHV-6B DNAemia was not found in all 20 patients examined at week 1. As a control, HHV-6B DNAemia in 21 BMT patients at weeks 2, 3, and 4 was examined. In four of 21 BMT patients (19%), HHV-6B DNAemia was found in a serum sample. The differences of the probability for HHV-6B DNAemia between CBT and BMT were significant at week 2, week 3, and week 2 or 3. In CBT patients, positive HHV-6B DNAemia at week 3 was significantly associated with early skin rash (88% vs 14%). In contrast, positive HHV-6B DNAemia at week 2 was not associated with skin rash.
By using the same methodology, we studied the incidence of HHV-6A infection in 50 adults after CBT. In one patient (2%), HHV-6A DNA was detected in serum samples on days 14, 21, and 28 after CBT. In the remaining 49 patients, HHV-6A DNA was not detected. Fever, skin rash, and liver dysfunction in our patient were considered to be associated with HHV-6A infection. This is the first report studying the incidence and the clinical features of HHV-6A infection in the early period after CBT.
In the present study, we examined the incidences of HHV-6 infection in adults after CBT by a real-time quantitative PCR method on serum samples. Because our study included only small patient number, prospective large-scale studies are needed to determine the role of HHV-6 infection in CBT patients.
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