Neural stem/progenitor cell transplantation therapy for perinatal brain damage.
| Project/Area Number |
16591084
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Institute for Developmental Research, Aichi Human Service Center |
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Principal Investigator |
NAKANISHI Keiko Inst. Dev. Res., Dept. Perinatology, senior scientist, 周生期学部, 主任研究員 (50280813)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | prenatal brain damage / hypoxic-ischemic encephalopathy / hypoxia-ischemia model / neural stem / progenitor cells / neural cell death / chondroitin sulfate / glutamate toxicity / 細胞外マトリックス / 神経保護因子 / プロテオグリカン |
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| Research Abstract |
Perinatal hypoxia-ischemia (HI) remains a critical issue affecting 2-3 of 1000 live births in developed countries, with no effective treatments except brain hypothermia. This project is aimed to examine whether neural stem/progenitor cell (NSPC) transplantation therapy may have therapeutic effect for perinatal HI. We produced neonatal HI rats by exposure to hypoxia following ligation of the right common carotid artery, and injected NSPCs into the right ventricle 24 hours after hypoxia. There was no difference between vehicle-and NSPC-injected rats in the wet weight of the right cerebral hemisphere measured 7 days after injection. When the rats were treated with NSPC combined with chondroitinase, which digests glycosaminoglycans of chondroitin sulfate proteoglycan, cerebral damage was significantly reduced. There was no obvious effect in chondroitinase-treated rats without NSPCs. Although we need further investigation, there is a possibility that intracerebral injection of NSPCs with chondroitinase may become a useful therapy for neonatal HI. Next, to elucidate a candidate for preventing neuronal cell death, we cultured rat neocortical neurons and examined NMDA-induced excitatory neuronal cell death. Preadministration of a highly sulfated chondroitin sulfate (CS) preparation, CS-E, significantly reduced neuronal cell death induced by NMDA, but other CS preparations such as CS-A, CS-B, CS-C, and CS-D, did not. These results indicate that CS-E may be a good candidate for neural cell protection.
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Report
(4 results)
Research Products
(26 results)
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[Patent(Industrial Property Rights)] 特許権2006
Inventor(s)
中西圭子, 大平敦彦, 佐藤義朗, 前田 浩
Industrial Property Rights Holder
愛知県・生化学工業株式会社
Industrial Property Number
2006-139843
Filing Date
2006-05-19
Description
「研究成果報告書概要(和文)」より
Related Report
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[Patent(Industrial Property Rights)] 特許権2006
Inventor(s)
中西圭子, 大平敦彦, 佐藤義朗, 前田 浩
Industrial Property Rights Holder
愛知県・生化学工業株式会社
Industrial Property Number
2006-139813
Filing Date
2006-05-19
Description
「研究成果報告書概要(和文)」より
Related Report
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