| Project/Area Number |
16591319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Okayama University |
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Principal Investigator |
NAOMOTO Yoshio Okayama University, Graduate School of Medicine. Dentistry and Pharmaceutical Sciences, Assistant professor, 大学院・医歯薬学総合研究科, 助教授 (00237190)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUOKA Junji Okayama University, Medical and Dental Hospital, Assistant, 医学部・歯学部附属病院, 助手 (30332795)
TANAKA Noriaki Okayama University, Graduate School of Medicine. Dentistry and Pharmaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (10127566)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | heparanase / COX-2 / anaiogenesis / 浸潤 / 転移 |
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| Research Abstract |
Heparanase (HPA) is thought to play critical roles in invasion and metastasis of esophageal carcinomas. Cyclooxygenase-2 (COX-2) is a potent carcinogenic and angiogenic molecule that promotes tumor angiogenesis by prostaglandin E2 synthesis. We analyzed HPA and COX-2 expression in esophageal carcinoma, and found that HPA and COX-2 exhibited a similar expression pattern in esophageal tumor tissues. Moreover, expression of these molecules showed a significant correlation with high intratumoral microvessel density. In esophageal cancer cell lines transfected with the HPA-cDNA, upregulation of COX-2 mRNA and protein was detected by RT-PCR and western blotting. A COX-2 promoter assay showed that cyclic AMP responsible element (CRE), NFκB and NF-IL-6 sites in the COX-2 promoter were essential for the transcriptional activation induced by HPA. Next, we studied the relation of HPA and COX-2 using breast cancer and gastric cancer. Our results suggest that HPA and COX-2, both of which are thought to play independently critical roles in tumor progression and metastasis, are closely related.
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