Study of inhibition of the metastasis in gastrointestinal carcinoma targeting for RANTES receptor
| Project/Area Number |
16591333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
FURUHATA Tomohisa Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (80359992)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Koichi Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (50136959)
TSURUMA Tetsuhiro Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (80381268)
KIMURA Yasutoshi Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (80311893)
前佛 均 札幌医科大学, 医学部, 助手 (90372820)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Gastric carcinoma / Colorectal carcinoma / Pancreas carcinoma / Invasion / Stroma cell / RANTES / MMP / HGF / 乳癌 / MMP-9 / VEGF-A / 単核球 / 浸潤能 / CCR |
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| Research Abstract |
In the present study, we investigated the effect of the RANTES-mediated interaction between carcinoma cell line (gastric, colon and pancreas carcinoma) and mononuclear cell line (THP-1) in tumor progression. RANTES production in THP-1 under co-culture with carcinoma cell line was higher than in those under mono-culture. RANTES receptors were expressed in THP-1, but not in those cancer cell lines; therefore it was suggested that RANTES might affect but not cancer cells. Matrix metalloproteinase (MMP)-9 expression in THP-1 was examined. Similar to RANTES production, MMP-9 expression in THP-1 under co-culture with carcinoma cell line was higher than in those under mono-culture. Invasion assays of cancer cell lines were performed. Cancer cells cultured with THP-1 invaded into Matrigel more frequently than those without THP-1. This invasive activity was inhibited to about 50% by an anti-RANTES antibody. These results suggested that tumor cells might acquire the potential for invasion by cooperating with THP-1 mediated by RANTES and other factors might play an important role in the interplay between tumor cells and THP-1. Therefore we carried out the expression analysis of carcinoma cell lines and THP-1 by Microarray method. In this experiment, the expression of HGF, LTB and MMP-2 was up-regulated under the condition of co-culture. We will investigate whether these phenomena are observed in vivo using resection specimen in future
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Report
(4 results)
Research Products
(7 results)
