| Project/Area Number |
16591355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The Jikei University School of Medicine |
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Principal Investigator |
TORIUMI Yasuo The Jikei University School of Medicine, Lecturer, 医学部, 講師 (20217590)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Yoshio The Jikei University School of Medicine, Lecturer, 医学部, 講師 (00246373)
TAKADA Koji The Jikei University School of Medicine, Assistant Professor, 医学部, 助教授 (30179452)
浦島 充佳 東京慈恵会医科大学, 医学部, 講師 (80203602)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | E2-EPF / Gastric Cancer / Ubiquitin |
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| Research Abstract |
1)RT-PCR
Gastric carcinoma cell lines (MKN1,MKN45,ECC10,GT3TkB) were examined for gene expression of E2-EPF using RT-PCR. mRNA expression of E2-EPF was observed in all of them.
2)Immunoblotting
Gastric carcinoma cell lines (MKN7,MKN45,IM95) and esophageal carcinoma cell line (KYSE 50) were analyzed by SDS-PAGE, followed by immunoblotting using an anti-E2-EPF polyclonal antibody of ab4504,and AP2120a. A single band of 24kDa was present in KYSE 50 and IM95 by using ab4504. But a single band of 48kDa was present in MKN7 and MKN45 by using ab4505. Various bands of muriti-ubiquitin chain conjugated with E2-EPF were ditected in each sample by using AP2120a. These result suggested that a double quantity body of E2-EPF might be present in gastric cancer.
3)Immunohistochemistry
Using paraffin-embedded specimens from patients with gastric cancer, E2-EPF protein was detected using an anti-E2-EPF polyclonal antibody of AP2120b using a dextran polymer conjugate two step visualization system. E2-EPF was enriched on the surface of nuclei in cancer cells. E2-EPF protein expression patterns within cancerous lesions were not associated with any of the following variables : TNM classification, stage, invasion into lymphatic or blood vessels, intramural metastasis, or histologic grades of differentiation.
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