| Project/Area Number |
16591391
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
WASHIYAMA Naoki Hamamatsu univ., sch.of med.the 1^<st> surgery, Assistant, 医学部, 助手 (40334982)
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| Co-Investigator(Kenkyū-buntansha) |
KAZUI Teruhisa Hamamatsu univ., sch.of med.the 1^<st> surgery, Professor, 医学部, 教授 (20094203)
SUZUKI Takayasu Hamamatsu univ., sch.of med.the 1^<st> surgery, Research assistant, 医学部, リサーチアシスタント (50397395)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Total arch replacement / Antegrade selective celebral perfision / Brain protection / Cerebral infarction / Edaravone / SEP / Hexanoyl-lysine / Malondialdehyde / Cerebral protection / Hexanol-lysine / MDA / 8-OHdG |
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| Research Abstract |
Objective : Reactive free radical species are thought to be involved in postoperative neurological dysfunction after antegrade selective cerebral perfusion (ASCP) in brain with old infarction. We assessed the brain protective effect of prophylactically administered edaravone, a free radical scavenger, for ASCP in brains with or without old infarction in a canine model.
Methods : Canine model of old cerebral infarction was created by injecting cylindrical silicone embolus into the middle cerebral artery. Animals showing obvious neurologic deficits and surviving 4 weeks or longer were included in the model. Deep hypothermia with ASCP was performed in both intact (non-edaravone; group A, edaravone-treated; group B) and infarcted animals (non-edaravone; group C, edaravone-treated; group D). Serum concentrations of malondialdehyde (MDA), hexanoyle-lysine (HEL), glutamate, and venous-arterial lactate difference (VALD) were measured and central conduction time (CCT) and amplitude of somatosensory evoked potentials were assessed during the operation.
Results : Compared with the intact groups, serum concentrations of MDA and HEL in group C significantly increased at the end of ASCP, while that of glutamate did so in the rewarming phase. Increase in all these biochemical parameters were suppressed in group D. In group C, VALD was significantly greater in the rewarming phase at 28℃ compared with intact groups. Significant prolongation of post operative CCT and decrease in neuronal activity were detected in group C, both of which recovered in group D.
Conclusion : Prophylactic administration of edaravone exerted a significant protective effect against postoperative neurological dysfunction after ASCP in a canine model with old cerebral infarction.
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