Basic research of neural circuit formation after experimental spinal cord injury
| Project/Area Number |
16591454
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka City University |
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Principal Investigator |
TAKAMI Toshihiro Osaka City University, Graduate School of Medicine, Lecturer, 大学院・医学研究科, 講師 (20305626)
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| Co-Investigator(Kenkyū-buntansha) |
OHATA Kenji Osaka City University, Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (70194264)
TSUYUGUCHI Naohiro Osaka City University, Graduate School of Medicine, Lecturer, 大学院・医学研究科, 講師 (50295705)
ADACHI Yoshiaki Kanazawa Institute of Technology, Applied Electronics Laboratory, Research Associate, 先端電子技術応用研究所, 助手 (80308585)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Spinal cord injury / Neural circuit / Functional recovery / Cell transplantation / Schwann cell |
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| Research Abstract |
Experimental spinal cord injury (SCI) was studied to better understand the process of neural circuit formation. Using a stereotactic device, an acute compression of mid-thoracic spinal cord of adult rat was developed. The rats were divided into several experimental groups, including control group of epidural injury, spinal tissue scarring group of subdural injury and treatment group of Schwann cell transplantation. Functional analysis included open field locomotor test, motor coordination test, temperature-induced pain response, magnetoencephalography (MEG) and positron emission tomography (PET), and was compared with imaging analysis.
Spinal tissue scarring at the injury site may cause a tethering effect on the spinal cord, which lead to significant alteration of the spinal cord parenchyma. Although the devastating effect of spinal tissue scarring on motor and sensory functions was not still resolved, the results suggested the importance of careful analysis in the treatment of spinal tissue scarring after SCI. Schwann cell transplantation was effective on promoting axonal sprouting and improving locomotor performance. PET suggested the decrease of glucose metabolism at the injury site although of its limitation of image clearance. MEG was used to determine the sensory evoked field under the condition of electrical stimulation of sciatic nerve. The technical limitations, including magnetic shield, the intensity of electrical stimulation or sensitivity, need to be further analyzed.
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Report
(3 results)
Research Products
(13 results)
