Bone and cartilage regeneration by using fat tissue
| Project/Area Number |
16591494
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Yamaguchi University |
|---|
Principal Investigator |
TANAKA Hiroshi Yamaguchi University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (50197464)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
|
|---|
| Keywords | fat tissue / adipocyte / chondrocyte / bone formation / cartilage regeneration / gene expression |
|---|
| Research Abstract |
Differentiation for osteoblast
Cultured cells from fat tissue were treated with dexamethasone or growth factors. Cells were determined for alkaline phosphatase activity and analyzed by RT-PCR for osteoblast markers. Alkaline phosphatase-positive cells were present in cells treated with dexamethasone or bone morphogenetic protein. Gene expression for osteoblast markers were increased.
Differentiation for chondrocyte
Cultured cells from fat tissue were cultured in three-dimensional structure, such as pellet or micromass. Dexamethasone or growth factors were added for three weeks. Histological and immunohistochemical analysis of pellet cultures revealed cartilage-like tissue characterized by metachromatically stained extracellular matrix and type II collagen immunoreactivity, indicative of chondrogenesis
|
Report
(3 results)
Research Products
(2 results)
