The role of ubiquitination in signal transduction of Osteosarcoma
| Project/Area Number |
16591501
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
YOKOUCHI Masahiro Kagoshima University, orthopaedics, assistant, 大学院医歯学総合研究科, 助手 (80359976)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Kyouji Kagoshima University, orthopaedics, lecturer, 医学部・歯学部附属病院, 講師 (50325784)
MATSUNAGA Shunji Kagoshima University, orthopaedics, lecturer, 医学部・歯学部附属病院, 講師 (90229500)
YONE Kazunori Kagoshima University, orthopaedics, assistant professor, 大学院医歯学総合研究科, 助教授 (40182844)
小宮 節郎 鹿児島大学, 大学院医歯学総合研究科, 教授 (30178371)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
|
|---|
| Keywords | osteoclast / osteosarcoma / signal transduction / inhibitor / ubiquitin system / hyaluronan / Src / Cbl |
|---|
| Research Abstract |
Several studies have suggested that increased production of hyaluronan (HA) is associated with metastatic behavior in various malignant tumors. To our knowledge, HA molecular weights required for metastasis are still remained to be unsolved in osteosarcoma. We examined the size of HA and hyaluronan synthase (HAS) isoforms related to biological functions required for metastasis in a stably highly metastatic osteosarcoma cell line, LM8. We found that HA of molecular weight which HAS3 produces enhanced biological functions related to metastasis such as cell proliferation, invasion, and degradation of extracellular matrix. We also found that cell proliferation and invasion were inhibited by suppressing the activity of HAS3 expressed in LM8 cells, using hyaluronan synthase suppressor, 4-methylumbelliferone (MU). Moreover, HA with the molecular weight related to HAS2 was the most adherent to CD44 in LM8 cells, suggesting that HAS2 may play an important role in pericellular coat formation. These results suggest that HAS3-related HA enhances crucial biological activities necessary for metastasis and that HAS2-related HA offers an advantageous environment for osteosarcoma cells.
|
Report
(3 results)
Research Products
(7 results)
