| Co-Investigator(Kenkyū-buntansha) |
TOYAMA Yoshiaki Keio University, School of Medicine, Professor, 医学部, 教授 (40129549)
AIZAWA Mamoru Meiji University, Department of Industrial Chemistry, Associate Professor, 理工学部, 助教授 (10255713)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
The aim of this study is to develop novel scaffolds for hard-tissue regeneration from apatite single-crystal fibers. We clarified a relationship between the properties of the resulting scaffolds and the cellular responses of osteoblasts cultured using the scaffolds.
In 2003, We have done some studies listed below to evaluate the usefulness of apatite fiber scaffolds as a scaffold of osteoblasts in tissue engineering of bone.
1.Posterolateral fusion was conducted in rats using apatite fiber mesh loaded with rhBMP-2. within 2 months after the implantation, lumbar spines were excised and the fusion status was evaluated both radiologically and histologically.
2.Bone marrow cells were seeded and cultured on/in three types of apatite fiber scaffolds with different porosity and pore sizes. After about 2 week's subculture, cells/scaffold composites were implanted into the subcutaneous tissue of athymic mise and excised within 3 months after the surgery for histological examination to determine th
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e extent of ossification.
In experiment 1, we have examined that the percentage of fusion rate was almost 100%, by using apatite fiber scaffolds combined with rhBMP-2, and concluded that the scaffolds were effective as a carrier of rh-BMP-2.
In experiment 2, the cells cultured in AFS induced successful ossification, and found that AFS could be useful as a scaffold for tissue engineering of bone. Scaffolds with higher porosity, had a tendency to achieve more pronounced ossification.
However, as these scaffolds had a high porosity of 98-99%, we needed to operate carefully the scaffolds on handling during in vivo/vitro operation. In 2004, in order to enhance the mechanical properties of the scaffolds, we partly modified the processing of scaffolds utilizing 1. cross-linking of I-collagen with apatite fiber, and 2. precipitation of apatite gel on the apatite fiber, and then successfully create the strengthened scaffolds. The resulting scaffolds showed good cellular responses : initial cell attachment, proliferation and differentiation into osteoblasts. Less
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