| Project/Area Number |
16591598
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TSUCHIDA Masahiro Yamaguchi University, Faculty of Medicine, Assistant Professor, 医学部附属病院, 講師 (80325216)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAI Kimio Yamaguchi University, Faculty of Medicine, Assistant Professor, 医学部附属病院, 講師 (10284241)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Geranylgeranylacetone / Jurkat T cell / apoptosis / Heat shock protein (HSP) / ischemic / reperfusion injury / Jurkat T cell / camptothecine / 熱ショック / heat shock protein(HSP) / geranylgeranylacetone |
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| Research Abstract |
Recently geranylgeranylacetone (GGA), an anti gastro-duodenal ulcer drug, has been demonstrated to induce activation of heat shock proteins (HSPs) and renders protection apoptosis in vivo and vitro. However, the molecular mechanisms of anti-apoptotic effects of GGA remain undefined. Thus, we investigated the effect of GGA on camptothecin-induced apoptosis of Jurkat T cells.
Agarose-gel electrophoresis showed a typical DNA ladder when the Jurkat cells were exposed to canptothecin at 50 μM for 4 hours. The DNA ladder formation was inhibited, when the cells were pretreated with GGA at 5 μM for 4 hours. Then, the cells were analyzed by Western blotting. Caspase-3,caspase-9 and PARP were cleaved in the cells exposed to camptothecin only. The cells treated with GGA at 5 μM for 4 hours markedly increased the expression of heat shock protein 70 (HSP70) and decreased the camptothecin-induced cleavage of caspase-3,caspase-9 and PARP. These results demonstrate that GGA induced HSP70 and renders protection camptothecin-induced apoptosis in Jurkat cells.
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