Classification of mechanism of androgen independency in prostate cancer
| Project/Area Number |
16591606
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
MASUMORI Naoya Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (20295356)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAMOTO Taiji Sapporo Medical University, School of Medicine, Professor, 医学部, 教授 (50112454)
TAKAHASHI Atsushi Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (20274946)
KATO Kazunori Sapporo Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (60233780)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | prostate cancer / neuroendocrine cell / metastasis / invasion / gelsolin |
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| Research Abstract |
<Background> : Although neuroendocrine (NE) cells in prostate cancer have been speculated to accelerate the growth and progression of surrounding cancer cells, direct evidence is insufficient. We investigated the effect of an NE allograft (NE-10) and its cell line, NE-CS, which were established from the prostate of the LPB-Tag 12T-10 transgenic mouse, on the human prostate cancer cell line LNCaP.
<Method>s : The proliferation and pulmonary metastasis of LNCaP xenografts in athymic mice with and without NE-10 allografts were evaluated. Boyden chamber assay and microarray analysis was performed to investigate change in invasion/migration and mRNA of LNCaP cells under the influence of the NE cells, respectively.
Results : The NE-10 did not influence the proliferation of the LNCaP. The pulmonary metastasis of LNCaP with the NE-10 significantly increased compared to mice without it. The NE-CS cells accelerated the in vitro invasion/migration of adenocarcinoma cells. Increased expression of mRNA of gelsolin in LNCaP cells under the incubation with the supernatant of NE-CS cells was observed.
<Conclusion>s : The NE-10 allograft promotes pulmonary metastasis of subcutaneously inoculated LNCaP cells by facilitating cell invasion. Secretions from NE cells up-regulated expression of gelsolin, which is an actin-binding protein and resulted in acceleration of the migration of LNCaP cells.
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Report
(3 results)
Research Products
(11 results)
