The role of cell cortex structures in mechanical stress signal transduction in osteoblasts.
| Project/Area Number |
16591822
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
IKEGAME Mika Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (70282986)
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| Co-Investigator(Kenkyū-buntansha) |
EJIRI Sadakazu Niigata Univ., Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (40160361)
YAMAMOTO Toshio Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30107776)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | mechanical stress / osteoblasts / cytoskeleton / cell cortex / ultrastructure / histochemistr / actin / bone suture |
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| Research Abstract |
Tensile stress (TS) has been shown to stimulate osteogenesis in the cranial suture of mouse calvaria. In order to clarify the role of the adhesion complex and cytoskeleton in this mechanism, the morphological changes in such stressed suture cells were investigated.
TS was applied to the cranial suture of neonatal mouse calvaria in culture. Some were stained with Alexa phalloidin and their actin fibers observed with confocal laser scanning microscopy, with others being observed with TEM. The results show that in the mature osteoblasts in the suture, actin was accumulated in the cell cortex. In the preosteoblastic cells and fibroblastic cells, actin was observed as stress fiber-like structures. Those were thicker in fibroblastic cells than in the preosteoblastic cells. With the tensile stress for 3 or 6 hours, all the cells in the sutures were stretched and elongated in the direction of the tensile stress. Morphological change of actin-cytoskeleton was observed consistent with the change of the cell shape. Thickness of the actin stress fiber-like structure and actin cell cortex were increased in the cells of the tensile stressed suture for 6 hours, but they were not clearly increased in the 3 hour-stressed group. Cadherin, one of the cell adhesion molecules, was observed along the cell cortex of osteoblasts at any experimental time course. On the other hand, immunological activities of β-catenin and vinculin became intense along the cell cortex in the osteoblasts of the tensile stressed groups.
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Report
(3 results)
Research Products
(2 results)
