| Project/Area Number |
16591824
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
OKAMOTO Shigefumi Fukuoka Dental College, Faculty of Dentistry, Lecturer, 歯学部, 講師 (50311759)
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| Co-Investigator(Kenkyū-buntansha) |
KAWABATA Shigetada Osaka University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (50273694)
NAKAGAWA Ichiro Osaka University, Graduate School of Dentistry, Assistant Professor, 大学院・歯学研究科, 講師 (70294113)
HAMADA Shigeyuki Nihon University, Advanced Research Institute for the Sciences and Humanities, Professor, 大学院・総合科学研究科, 教授 (60028777)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Steptococcus sobrinus / water-insoluble α-glucans / inflammatory immune responses / periodontal diseases / 遊走能 / 活性酸素 / レンサ球菌 / 免疫応答 |
|---|
| Research Abstract |
Water-insoluble α-glucans (WIG) are synthesized from sucrose by the glucosyltransferase-I of mutans streptococci, that play an important role in the development of dental plaque. We found that WIG synthesized by Streptococcus sobrinus activated mouse peritoneal exudate macrophages to produce cytokines. The immunological responses were not due to contamination by sucrose, water-soluble glucan, lipopolysaccharide, or peptidoglycan. WIG stimulated human monocytes to produce TNF-α and IL-8. Human polymorphonuclear cells were also activated by WIG, resulting in chemotaxis of the cells and production of hydrogen peroxide. These results suggest that WIG modulate macrophage- and granulocyte-induced inflammatory immune responses. Furthermore, since WIG injured human oral squamous cells directly, we speculate that oral squamous cell injury and inflammation induced by α-glucans are associated with the development of periodontal diseases.
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