Study for regulatory mechanism for atrophy of tongue and masticatory muscles by the system of model mouse

• Project/Area Number: 16591871
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Tsurumi University
• Principal Investigator: YAMASE Akira Tsurumi University, School of Dental Medicine, Department of Pharmacology, Lecturer, 歯学部, 講師 (20166763)
• Co-Investigator(Kenkyū-buntansha): ASADA Yoshinobu Tsurumi University, School of Dental Medicine, Department of Pediatric Dentistry, Professor, 歯学部, 教授 (20184145) ; OHNUKI Yoshiki Tsurumi University, School of Dental Medicine, Department of Physiology, Assistant, 歯学部, 助手 (50288114)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,100,000 (Direct Cost: ¥3,100,000); Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: atrophy / hypertrophy / masseter / tongue / growth factors / synaptogenesis / muscle satellite cell / muscle dystrophy / IGF / IGFR / 咬筋 / 腓腹筋 / Klothoマウス / 老化 / モデルマウス / 小眼球症マウス / mitf遺伝子 / AChR除去 / AChR転換 / 咀嚼 / Miマウス

Research Abstract

The following new findings were obtained in the present study.
1. The regulatory program for myogenesis of tongue is similar to that for limb myogenesis other than head myogenesis.
2. Myogenesis and synaptogenesis in the masticatory muscles are delayed in comparison with other muscles and are not completed even at birth, whereas the development of tongue muscles proceeds faster than those of other muscles and ends at around birth.
3. Insulin-like growth factors, their receptors and binding proteins are involved in the atrophy of mouse masseter muscle induced by the transition from hard to soft diets.
4. The pool size of satellite cells in the masseter muscle is greater than those in other muscles such as gastrocnemius, soleus and diaphragm muscles of mdx mouse and the pool size is one factor for determining the extent of myofiber degeneration.
5. Transforming growth factor β_8 and myostatin function as negative regulators for the hypertrophy induced by clenbuterol, a β_2 adrenergic agonist, whereas Insulin-like growth factors work as positive regulators.

Research Products

• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement of teeth (2007)
  • Author(s): LUAN, X., ITO, Y., HOLLIDAY, S., WALKER, C., DANIELL, J., GALANG, T.M., FUKUI, T., YAMANE, A., BEGOLE, E., EVANS, C., DIEKWISCH, T.G.H.
  • Journal Title: J. Histochem. Cytochem. 55・2 Pages: 127-140
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement of teeth (2007)
  • Author(s): LUAN, X., ITO, Y., HOLLIDAY, S., WALKER, C., DANIELL, J., GALANG, T.M., FUKUI, T.YAMANE, A., BEGOLE, E., EVANS, C., DIEKWISCH, T.G.H.
  • Journal Title: J. Histochem. Cytochem. 55(2) Pages: 127-140
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Extracellular matrix-mediated tissue remodeling following axial movement of teeth (2007)
  • Author(s): LUAN, X., ITO, Y., HOLLIDAY, S., WALKER, C., DANIELL, J., GALANG, T.M., FUKUI, T., YAMANE, A., BEGOLE, E., EVANS, C., DIEKWISCH,T.G.H.
  • Journal Title: J.Histochem. Cytochem. 55・2 Pages: 127-140
• [Journal Article] Transforming growth factor β_s are up-regulated in the rat masseter muscle hypert rophied by clenbuterol, a β_2 adrenergic agonist (2006)
  • Author(s): AKUTSU, S., SHIMADA, A., YAMANE, A.
  • Journal Title: Brit J. Pharmacol. 147(4) Pages: 412-421
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The expressions of insulin-like growth factors, their receptors, and binding proteins are related to the mechanism regulating masseter muscle mass in the rat (2006)
  • Author(s): MATSUMOTO, T., AKUTSU, S., WAKANA, N., MORITO, M., SHIMADA, A, YAMANE, A.
  • Journal Title: Archs. oral Biol. 51・7 Pages: 603-611
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transforming growth factor βs are up-regulated in the rat masseter muscle hypertrophied by clenbuterol, a β2 adrenergic agonist (2006)
  • Author(s): AKUTSU, S., SHIMADA, A., YAMANE, A.
  • Journal Title: Brit J. Pharmacol. 147(4) Pages: 412-421
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The expressions of insulin-like growth factors, their receptors, and binding proteins are related to the mechanism regulating masseter muscle mass in the rat (2006)
  • Author(s): MATSUMOTO, T., AKUTSU, S., WAKANA, N., MORITO, M., SHIMADA, A, YAMANE, A.
  • Journal Title: Archs. oral Biol. 51(7) Pages: 603-611
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transforming growth factor βs are up-regulated in the rat masseter muscle hypertrophied by clenbuterol, a β_2 adrenergic agonist (2006)
  • Author(s): AKUTSU, S., SHIMADA, A., YAMANE, A.
  • Journal Title: Brit J.Pharmacol. 147・4 Pages: 412-421
• [Journal Article] The expressions of insulin-like growth factors, their receptors, and binding proteins are related to the mechanism regulating masseter muscle mass in the rat (2006)
  • Author(s): MATSUMOTO, T., AKUTSU, S., WAKANA, N., MORITO, M., SHIMADA, A, YAMANE,A.
  • Journal Title: Archs. oral Biol. 51・7 Pages: 603-611
• [Journal Article] Transforming growth factor βs are up-regulated in the rat masseter muscle hype rtrophied by clenbuterol, a β_2 adrenergic agonist (2006)
  • Author(s): AKUTSU, S., SHIMADA, A., YAMANE, A.
  • Journal Title: Brit J.Pharmacol. 147(4) Pages: 412-421
• [Journal Article] The expressions of insulin-like growth factors, their receptors, and binding proteins are related to the mechanism regulating masseter muscle mass in the rat (2006)
  • Author(s): MATSUMOTO, T., AKUTSU, S., WAKANA, N., MORITO, M., SHIMADA, A., YAMANE.A.
  • Journal Title: Archs. oral Biol. (in press)
• [Journal Article] Growth factors and proliferation of cultured rat gingival cells in response to Cyclosporin A (2005)
  • Author(s): YOSHIDA, T., NAGATA, J., YAMANE, A.
  • Journal Title: J. Periodont. Res 40(1) Pages: 11-19
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Amelogenin sequence and enamel biomineralization in Rana pipiens. (2005)
  • Author(s): WANG, X., ITO, Y., LUAN, X., YAMANE, A., DIEKWISCH, D.G.H.
  • Journal Title: J. Exp. Zool. (Mol. Dev. Evol.) 34B Pages: 1-10
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Bones, teeth, and genes : A genomic homage to Harry Sicher's"axial movement of teeth" (2005)
  • Author(s): HOLLIDAY, S., SCHNEIDER, B., GALANG, M.T., FUKUI, T., YAMANE, A., LUAN, X., DIEKWISCH, D.G.H.
  • Journal Title: World J. Orthod. 6(1) Pages: 301-310
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damages in mdx mice (2005)
  • Author(s): YAMANE, A., AKUTSU, S., DIEKWISCH, T.G.H., MATSUDA, R.
  • Journal Title: Am. J. Physiol. : Cell Physiol. 289(1)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effect of stress response to surgical procedures upon secretion of salivary immun oglobulin A in mice (2005)
  • Author(s): WATANABE, N.Y., NAKAGWA, Y., AKUTSU, S., YAMANE, A.
  • Journal Title: Oral Sci. Intl. 2(2) Pages: 96-103
  • NAID: 10020401619
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Embryonic and postnatal development of masticatory and tongue muscles (2005)
  • Author(s): YAMANE, A.
  • Journal Title: Cell Tissue Res. 322(2) Pages: 183-189
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Growth factors and proliferation of cultured rat gingival cells in response to Cyclosporin A (2005)
  • Author(s): YOSHIDA, T., NAGATA, J., YAMANE, A.
  • Journal Title: J. Periodont. Res. 40(1) Pages: 11-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Amelogenin sequence and enamel biomineralization in Rana pipiens. (2005)
  • Author(s): WANG, X., ITO, Y., LUAN, X., YAMANE, A., DIEKWISCH, D.G.H.
  • Journal Title: J. Exp. Zool. Mol. Dev. Evol. 304(2) Pages: 177-186
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bones, teeth, and genes : A genomic homage to Harry sicher's "axial movement of teeth" (2005)
  • Author(s): HOLLIDAY, S., SCHNEIDER, B., GALANG, M.T., FUKUI, T., YAMANE, A., LUAN, X., DIEKWISCH, D.G.H.
  • Journal Title: World J. Orthod. 6(1) Pages: 301-310
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damages in mdx mice (2005)
  • Author(s): YAMANE, A., AKUTSU, S., DIEKWISCH, T.G.H., MATSUDA, R.
  • Journal Title: Am. J. Physiol. Cell Physiol. 289(1)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of stress response to surgical procedures upon secretion of salivary immunoglobulin A in mice (2005)
  • Author(s): WATANABE, N.Y., NAKAGWA, Y., AKUTSU, S., YAMANE, A.
  • Journal Title: Oral Sci. Intl. 2(2) Pages: 96-103
  • NAID: 10020401619
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bones, teeth, and genes : A genomic homage to Harry Sicher's "axial movement of teeth" (2005)
  • Author(s): HOLLIDAY, S., SCHNEIDER, B., GALANG, M.T., FUKUI, T., YAMANE, A., LUAN, X, DIEKWISCIL D.G.H.
  • Journal Title: World J.Orthod. 6(1) Pages: 301-310
• [Journal Article] Growth factors and proliferation of cultured rat gingival cells in response to Cyclosporin A (2005)
  • Author(s): YOSHIDA, T., NAGATA, J., YAMANE, A.
  • Journal Title: J.periodont.Res. 40(1) Pages: 11-19
• [Journal Article] Amelogenin sequence and enamel biomineralization in Rana pipiens. (2005)
  • Author(s): WANG, X., ITO, Y., LUAN, X., YAMANE, A., DIEKWISCH, D.G.H.
  • Journal Title: J.exp.Zool.(Mol.Dev.Evol.) 304(2) Pages: 177-186
• [Journal Article] Satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damages in mdx mice (2005)
  • Author(s): YAMANE, A., AKUTSU, S., DIEKWISCH, T.G.H., MATSUDA, R.
  • Journal Title: Am.J.Physiol. : Cell Physiol. 289(1)
• [Journal Article] Effect of stress response to surgical procedures upon secretion of salivary immunoglobulin A in mice (2005)
  • Author(s): WATANABE, N.Y., NAKAGWA, Y., AKUTSU, S., YAMANE, A.
  • Journal Title: Oral Sci.Intl. 2(2) Pages: 96-103
  • NAID: 10020401619
• [Journal Article] Bones, teeth, and genes : A genomic homage to Harry Sicher's "axial movement of teeth" (2005)
  • Author(s): HOLLIDAY, S., SCHNEIDER, B., GALANG, M.T., FUKUI, T., YAMANE, A., LUAN, X., DIEKWISCH, D.G.H.
  • Journal Title: World J.Orthod. 6(1) Pages: 301-310
• [Journal Article] Amelogenin sequence and enamel biomineralization in Rana pipiens. (2005)
  • Author(s): WANG, X., ITO, Y., LUAN, X., YAMANE, A., DIEKWISCH, D.G.H.
  • Journal Title: J.exp.Zool. (Mol.Dev.Evol.) 34B Pages: 1-10
• [Journal Article] Satellite cells and utrophin are not directly correlated with the degree of skeletal muscle damages in mdx mice (2005)
  • Author(s): YAMANE, A., AKUTSU, S., DIEKWISCH, T.G.H., MATSUDA, R.
  • Journal Title: Am.J.Physiol. : Cell Physiol. (in press)
• [Journal Article] Change from a hard to soft diet alters the expression of insulin-like growth factors, their receptors, and binding proteins in association with atrophy in adult mouse masseter muscle (2004)
  • Author(s): URUSHIYAMA, T., AKUTSU, S., MIYAZAKI, J.-I., FUKUI, T., DIEKWISCH.T.G.H., YAMANE.A
  • Journal Title: Cell Tissue Res. 315(1) Pages: 97-105
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Exogenous hepatocyte growth factor inhibits myoblast differentiation by inducing myf5 expression and suppressing myoD expression in an organ culture system of embryonic mouse tongue (2004)
  • Author(s): YAMANE, A., AMANO, O., URUSHIYAMA, T., NAGATA, J., AKUTSU, S., FUKUI, T.
  • Journal Title: Eur. J. Oral Sci. 112(2) Pages: 177-181
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of diet consistency on the expression of insulin-like growth factors, their receptors, and binding proteins during the development of rat masseter muscle soon after weaning (2004)
  • Author(s): SAITO, T., FUKUI, K., AKUTU, S., NAKAGAWA, Y., ISHIBASHI, K., NAGATA, J., SHULER, C.F., YAMANE, A.
  • Journal Title: Archs. Oral Biol. 49(10) Pages: 777-782
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Progress of cell proliferation in striated muscle tissues during the development of mouse tongue (2004)
  • Author(s): NAGATA, J., YAMANE, A
  • Journal Title: J. Dent. Res. 83(12) : 926-929,2004 83(12) Pages: 926-929
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Effects of exogenous bone morphogenetic protein 2 on the formation of mouse molar tooth germ (2004)
  • Author(s): FUKUI, T., AKUTSU, S., HIRASHITA, A., OIDA, S., DIEKWISCH T.G.H., YAMANE, A.
  • Journal Title: J. Oral Biosci. 46 46(6) Pages: 530-535
  • NAID: 110003166654
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Change from a hard to soft diet alters the expression of insulin-like growth factors, their receptors, and binding proteins in association with atrophy in adult mouse masseter muscle (2004)
  • Author(s): URUSHIYAMA, T., AKUTSU, S., MIYAZAKI, J.-I., FUKUI, T., DIEKWISCH, T.G.H., YAMANE.A.
  • Journal Title: Cell Tissue Res. 315(1) Pages: 97-105
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Exogenous hepatocyte growth factor inhibits myoblast differentiation by inducing myf5 expression and suppressing myoD expression in an organ culture system of embryonic mouse tongue (2004)
  • Author(s): YAMANE, A., AMANO, 0., URUSHIYAMA, T., NAGATA, J., AKUTSU, S., FUKUI, T., DIEKWISCH, T.G.H.
  • Journal Title: Eur. J. oral Sci. 112(2) Pages: 177-181
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of diet consistency on the expression of insulin-like growth factors, their receptors, and binding proteins during the development of rat masseter muscle soon after weaning (2004)
  • Author(s): SAITO, T., FUKUI, K., AKUTU, S., NAKAGAWA, Y., ISHIBASHI, K., NAGATA, J.SHULER, C.F., YAMANE, A.
  • Journal Title: Archs. oral Biol. 49(10) Pages: 777-782
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Progress of cell proliferation in striated muscle tissues during the development of mouse tongue (2004)
  • Author(s): NAGATA, J., YAMANE, A.
  • Journal Title: J. Dent. Res. 83(12) Pages: 926-929
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of exogenous bone morphogenetic protein 2 on the formation of mouse molar tooth germ (2004)
  • Author(s): FUKUI, T., AKUTSU, S., HIRASHITA, A., OIDA, S., DIEKWISCH T.G.H., YAMANE, A.
  • Journal Title: J. Oral Biosci. 46(6) Pages: 530-535
  • NAID: 110003166654
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Change from a hard to soft diet alters the expression of insulin-like growth factors, their receptors, and binding proteins in association with atrophy in adult mouse masseter muscle (2004)
  • Author(s): URUSHIYAMA, T., AKUTSU, S., MIYAZAKI, J.-I., FUKUI, T., DIEKWISCH, T.G.H., YAMANE, A
  • Journal Title: Cell Tissue Res. 315(1) Pages: 97-105
• [Journal Article] Exogenous hepatocyte growth factor inhibits myoblast differentiation by inducing myf5 expression and suppressing myoD expression in an organ culture system of embryonic mouse tongue (2004)
  • Author(s): YAMANE, A., AMANO, O., URUSHIYAMA, T., NAGATA, J., AKUTSU, S., FUKUI, T.
  • Journal Title: Eur.J. oral Sci. 112(2) Pages: 177-181
• [Journal Article] Effects of diet consistency on the expression of insulin-like growth factors, their receptors, and binding proteins during the development of rat masseter muscle soon after weaning (2004)
  • Author(s): SAITO, T., FUKUI, K., AKUTU, S., NAKAGAWA, Y., ISHIBASHI, K., NAGATA, J., SHULER, C.F., YAMANE
  • Journal Title: Archs. oral Biol. 49(10) Pages: 777-782
• [Journal Article] Progress of cell proliferation in striated muscle tissues during the development of mouse tongue (2004)
  • Author(s): NAGATA, J., YAMANE, A
  • Journal Title: J.dent.Res. 83(12):926-929, 2004 83(12) Pages: 926-929
• [Journal Article] Effects of exogenous bone morphogenetic protein 2 on the formation of mouse molar tooth germ (2004)
  • Author(s): FUKUI, T., AKUTSU, S., HIRASHITA, A., OIDA, S., DIEKWISCH T.G.H., YAMANE, A.
  • Journal Title: J. oral Biosci. 46 46(6) Pages: 530-535
  • NAID: 110003166654
• [Journal Article] 口腔組織発生における肝細胞増殖因子の機能 (2004)
  • Author(s): 天野 修, 山根 明
  • Journal Title: 口腔組織培養学会誌 13(2) Pages: 1-11
• [Journal Article] BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T.G.H., MORITO, M., YAMANE, A.
  • Journal Title: Cell and Tissue Res. (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-labial surface of the mandibular first molar in microphthalmic mouse
  • Author(s): TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Archs. Oral Biol. (In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] BMP2, BMP4, and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T.G.H., MORITO, M., YAMANE, A.
  • Journal Title: Cell and Tissue Res. (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of excess hard tissue occurring in the mesio-labial surface of the mandibular first molar in microphthalmic mouse
  • Author(s): TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Archs. Oral Biol. (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] BMP2,BMP4,and their receptors are expressed in the differentiating muscle tissues of mouse embryonic tongue
  • Author(s): SUGA, T., FUKUI, T., SHINOHARA, A., LUAN, X., DIEKWISCH, T.G.H., MORITO, M., YAMANE,A.
  • Journal Title: Cell and Tissue Res. (In press)
• [Journal Article] Characterization of excess hard tisse occurring in the mesio-labial surface of th mandibular first molar in microphthalmic mouse
  • Author(s): TOMOHIRO, T., YAMANE, A., ASADA, Y.
  • Journal Title: Archs. Oral Biol. (In press)