A change of the metal-specific active site structure of superoxide dismutase from Porphyromonas gingivalis by the mutation of Gly155Thr.
| Project/Area Number |
16591874
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
HIRAOKA B.Yukihiro Matsumoto Dental University, Graduate School of Oral Medicine, Professor, 大学院・歯学独立研究科, 教授 (20097512)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Teruhito Institute for Oral Science, Assistant Professor, 総合歯科医学研究所, 講師 (90302893)
奥村 茂樹 松本歯科大学, 歯学部, 助手 (80350825)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Reactive oxygen / SOD / cambialistic enzyme / Metallo enzyme / Oxidoreductase / Porphyromanas gingivalis / site-directed mutagenesis / EPR spectrum |
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| Research Abstract |
Mn-superoxide dismutases (SODs), Fe-SODs and cambialistic SODs, its include P.gingivalis (P.g.) SOD, have a large degree of sequence homology and X-ray structural similarity. Gly155 of P.g.-SOD is located about 11Å from active metal sites and is mostly conserved in aligned amino acid sequences of Mn-SODs, but is substituted for Thr in most Fe-SODs. In order to clarify the structural bases of the metal-specific activity, we analyzed using high-field electron paramagnetic resonance spectroscopy.
We found that Gly155Thr mutation changes the metal-specific activity drastically from a cambialistic type to close to Fe-specific type. Also the Gly155Thr mutant of P.g.-SOD changed the Mn(II) spectrum so that it closely resembled the spectrum of Mn-substituted Fe SOD from E.coli. It has been concluded that Gly155 had universality as one of the amino acid residue which determines the Mn specific activity and Thr on same position had universality as one of the amino acid residue which determines the Fe specifc activity on SODs.
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Report
(3 results)
Research Products
(6 results)
