| Project/Area Number |
16592077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Showa University |
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Principal Investigator |
OKAMATSU Yoshimasa Showa University Dental School, Periodontology, Assistant, 歯学部, 助手 (50286845)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Makoto Showa University Dental School, Periodontology, Assistant, 歯学部, 講師 (80186767)
MIYAZAWA Yasushi Showa University Dental School, Periodontology, Assistant, 歯学部, 講師 (90219775)
OHAZAMA Atsushi Showa University Dental School, Periodontology, Assistant, 歯学部, 講師 (40266169)
TAKIGUCHI Takashi Showa University Dental School, Periodontology, Assistant, 歯学部, 助手 (60317576)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Osteoclast / Chemokine / RANKL / Human Monocyte |
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| Research Abstract |
Osteoclasts (OC) are bone resorbing multinucleated giant cells that are derived from hemopoietic precursors of the monocyte-macrophage lineage. Osteoclastic bone resorption consists of multiple steps, including the differentiation of osteoclast precursors, the fusion of mononuclear cells to form mature OC, the activation of OC to resorb bone and the survival of activated. RANKL (Receptor Activator of NF-κB Ligand) is member of the TNF family and is one of key molecules that regulates both osteoclastogenesis and bone resorption. RANKL expression by osteoblasts as well as by activated T cells has been shown to regulate these processes. However, the participation of additional factors induced by RANKL stimulation is less well characterized.
Chemokines play an important role in immune and inflammatory responses by inducing migration and adhesion of leukocytes, and have also been reported to participate in the regulation of osteoclast (OC) differentiation from hematopoietic precursor cells of the monocyte-macrophage lineage. However, the effect of these chemokines as coupling factor between osteoclast and osteoblasts remain unclear. In this study, we can get these results until now.
1)RANKL-induced mature osteoclast from mouse bone marrow up-regulated the expression of MIP (Macrophage inflammatory protein)-1 gamma gene and CCR1 gene.
2)The signal tranceduction of MIP-1 gamma indicated via NF-kB.
3)RANKL-induced mature osteoclast from mouse bone marrow also up-regulated the expression of integrin beta7 gene and MIP-1 gamma related to expression of integrin beta7 gene in osteoclasts.
4)RANKL and M-CSF stimulated-CD14 positive human monocyte differentiated into mature osteoclasts, and these cells released MIP-1 alpha.
5)IL-1-stimulated osteoblasts from mouse calvaria produced RANTES and MIP-1 alpha.
These results suggest that many chemokines related osteoclast differentiation.
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