| Project/Area Number |
16592080
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Showa University |
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Principal Investigator |
OHAZAMA Atsushi Showa University, Periodontology, Lecturer, 歯学部, 講師 (40266169)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Ikkα / NF-kB / Notch / Tooth Development / Epithelial Invagination / Stellate Reticulum / Jagged / 外胚葉異形成症 / 咬頭形成 / TNF |
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| Research Abstract |
The NF-κB pathway plays a major role in many physiological and pathological processes. It has been shown that NF-κB and Ikkα are involved in tooth development. Evagination was observed in presumptive incisor region of Ikkα mutant mice. The downregulation of Notch have been found in Ikkα mutant incisor tooth. In wild-type, the expression of Notch were observed in the stellate reticulum (SR) of tooth bud epithelium while they were absent from the basal epithelial cells. The downregulation of Notch expression in Ikkα mutant incisor tooth germ reveal the possibility that the failure of the SR formation led to the loss of Notch expression in Ikkα mutant incisor. That prompt us to investigate whether the SR are presented in Ikkα mutant incisor or not. The SR marker, CD44 and SLUG expression were detected in the incisor epithelium of Ikkα mutant mice. The SR have been reported to play a critical role in enamel formation through Msx2 signalling. Msx2 and normal enamel deposition were detected in Ikkα mutant incisor tooth. These suggested that the SR were existed in Ikkα mutant incisor tooth. On the other hand, there have been no reports that describe the relationship between Ikkα and Notch. Ikkα mutant mice also showed cleft palate that were caused by unelevated palatal shelves and fusion between tongue and palatal shelves. Same phenotypes also have been found in Notch ligand, Jagged2 mutant mice. The data should be another evidence of the relationship between Ikkα and Notch during development. Ikkα/Notch are essential as kinase independent pathway in tooth development.
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