The roll of soluble interleukin-1 receptor type 2 for periodontal tissue
| Project/Area Number |
16592082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Aichi-Gakuin university |
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Principal Investigator |
ISHIHARA Yuichi Aichi-Gakuin university, dentistry, Lecturer, 歯学部, 講師 (50261011)
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| Co-Investigator(Kenkyū-buntansha) |
KOIDE Masanori Aichi-Gakuin university, dentistry, Lecturer, 歯学部, 講師 (10367617)
TANAKA Shigehisa Aichi-Gakuin university, dentistry, Lecturer, 歯学部, 講師 (30367619)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | soluble interleuklin-1receptor type 2 / gingival crevicular fluid / chronic periodontitis / interleukin-1β / ELISA / aggressive periodontitis / IL-1receptor type2 |
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| Research Abstract |
Head investigator and investigators have provided the results from this grant projects.
(1)One hundred and eighty one gingival crevicular fluids samples were obtained from 52 periodontitis patients and interleukin-1 (IL-1)β and soluble IL-1 receptor type 2 (sIL-1Rt2) were measured by using ELISA assay kits. The concentration of IL-1β and sIL-1t2 were positive correlated with the periodontal disease severity. On the other hand, GCF IL-1b and sIL-1t2 concentrations from chronic periodontitis (CP) patients and aggressive periodontitis (AgP) patients were also examined. IL-1β was no different between CP (82.2±12.3ng/ml)and AgP(68.3±9.4ng/ml), however, AgP sIL-1Rt2(3.0±0.6ng/ml) was significantly decreased compared with CP sIL-1Rt2(1.1±0.3ng/ml). Therefore, these results suggest that AgP disease character of rapid periodontal tissue breakdown may relate to the decreased sIL-1Rt2 production. (2)A total of 102 Japanese patients and 88 periodontally healthy volunteers were recruited at the Depa
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rtment of Periodontology in Aichi-gakuin University from 2002 to 2004. Polymorphisms were identified by direct sequence method. After the single nucleotide polymorphisms (SNPs) identification, allelo- and haplotype analysis were performed. A 14.5-kb genomic region containing all exons and relevant exon-intron boundaries of IL1R1 and IL1R2 were sequenced in 24 AgPs and 24 controls. Within genomic regions containing all exons and relevant exon-intron boundaries in IL1R1 and IL1R2,38 SNPs were identified in 48 subjects. Five were located within coding elements, 2 in the 5' flanking region,22 within introns, and 9 in the 3' untranslated region. Frequency of 3 SNPs and 3 haplotypes in IL1R2 showed significant differences between patients with AgP and controls (p<0.05). (3)Gingival fibroblasts were isolated from chronic periodontitis patients. And cells were stimulated with Actinobacillus actinomycetemcomitans lipopolysaccharide, which is strongly associated with AgP. IL-1β and IL-1Rt2 mRNA gene expression were measured. However, protein production was only measured for IL-1β and not for sIL-1Rt2 in culture supernatant. Project is now undergoing for different experimental design using immunocompetent cell such as neutrophil or mononuclear cells. Less
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Report
(3 results)
Research Products
(21 results)
