Analysis of genetic polymorphisms with Periodontitis in Down's syndrome

Research Project

Project/Area Number	16592102
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Social dentistry
Research Institution	Matsumoto Dental University
Principal Investigator	KASAHARA Hiroshi (2005) Matsumoto Dental University, Graduate school of Oral Medicine, Professor, 大学院・歯学独立研究科, 教授 (50097490) 穂坂一夫 (2004) Matsumoto Dental University, 大学院・歯学独立研究科, 助教授 (10199466)
Co-Investigator(Kenkyū-buntansha)	WANG Pao-Li Matsumoto Dental University, School of Dentistry, Professor, 歯学部, 教授 (20213613) 笠原浩松本歯科大学, 大学院・歯学独立研究科, 教授 (50097490)
Project Period (FY)	2004 – 2005
Project Status	Completed (Fiscal Year 2005)
Budget Amount *help	¥3,400,000 (Direct Cost: ¥3,400,000) Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000) Fiscal Year 2004: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywords	Down's syndrome / Periodontal disease / Polymorphism / PCR-RFLP
Research Abstract	It is famous that patients with Down's syndrome have a high risk of periodontal disease from an early age, but little is known about the genetic factors that may cause or exacerbate of this disease. A purpose of this study is to detect involving genes by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyzing. The subjects were 28 Japanese with Down's syndrome. Informed consent and oral screening were completed. Their DNAs were isolated from lingual mucosal cells and examined with the frequency of specific single nucleotide polymorphisms (SNPs) by PCR-RFLP method. The SNPs on which we focused were of the pro-inflammatory cytokine genes Interleukin (IL)-1A(-889) and IL-1B(+3953), and of the Toll-like receptor genes (TLR)2(Arg677Trp) and TLR4(Asp299Gly). As the result, in the place to the present, it wasn't possible to have found a significant difference in these SNPs and beta Integrins (posted as a paper). We continue to examine and analyze other cytokines, peptides and alexin-related molecules.