Visualization of the neural stem cells and application to the regenerative medicine
| Project/Area Number |
16606002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
幹細胞生物学
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| Research Institution | Kyoto University |
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Principal Investigator |
OHTSUKA Toshiyuki Kyoto University, Institute for Virus Research, Associate Professor, ウイルス研究所, 助教授 (20324709)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Development・Differentiation / Brain・Nervous system / Neuroscience / Regenerative medicine |
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| Research Abstract |
For the purpose of marking and purifying neural stem cells, we generated transgenic mice using promoters from Hes genes (pHes1 or pHes5) to drive expression of destabilized enhanced green fluorescent protein (d2EGFP). In the developing brains of the pHes1-d2EGFP transgenic mice, GFP expression was restricted to undifferentiated cells in the VZ during the embryonic period. Furthermore, the GFP expression was preferentially observed in cells with the capacity to self-renew in asymmetrical cell division in culture, indicating that they retain the capacity to self-renew. Our results suggested that pHes1-d2EGFP transgenic mice can be used to sort the neural stem cells by FACS and to explore similarities and differences among neural stem cells during development (Molecular and Cellular Neuroscience 2006 31:109-122).
Thus, we sorted the GFP-positive cells from the dorsolateral telencephalon (neocortical region) at different developmental stages, then performed gene expression profiling with GeneChip arrays, and evaluated the genes being differentially expressed at different embryonic stages. As the result of the extensive analysis of gene expression in neural stem cells, many candidate genes that were transitionally expressed during the course of development have been found. We have been examining their expression patterns in embryonic brains with in situ hybridization and analyzing the molecular functions in cortical development, and have identified some genes that exhibit unique expression patterns during brain development, could serve as a marker of specific developmental stage, and could be functionally involved in maintenance of neural stem cells or in neuronal/glial differentiation.
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Report
(3 results)
Research Products
(3 results)
