• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Unlock the biodiversity and functioning in food-webs using metabarcoding and metagenomics

Research Project

Project/Area Number 16F16092
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Biodiversity/Systematics
Research InstitutionKyoto University

Principal Investigator

本川 雅治  京都大学, 総合博物館, 教授 (30293939)

Co-Investigator(Kenkyū-buntansha) HE KAI  京都大学, 総合博物館, 外国人特別研究員
Project Period (FY) 2016-10-07 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2018: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2017: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2016: ¥800,000 (Direct Cost: ¥800,000)
Keywordsメタバーコーディング / 哺乳類 / 生物多様性 / 種分化 / 真無盲腸類 / 同所的分布 / 垂直分布 / DNAバーコーディング
Outline of Annual Research Achievements

本研究ではメタバーコーディングとメタゲノミクスの手法を用い,同所的に分布する真無盲腸類(食虫類)の食性と腸内微生物相の多様性解明を目指した.前年度までに収集したサンプルについて,その食性を明らかにするためのメタバーコーディングと同位体解析を行った.胃内容物から動物性および植物性のDNAを新たに抽出し,春,夏,秋の季節変化および標高(低,中,高)による違いを考慮した解析を行った.Illuminaプラットフォームを用いて,無脊椎動物についてはCOI,植物質についてはchloroplast trnl遺伝子をDNAバーコーディング領域とした.得られた配列データはスーパーコンピュータを用いて,既存のDNAバーコードデータとの比較を行い,真無盲腸類が食物として利用している無脊椎動物および植物質を明らかにした.腸内微生物相についてはメタゲノミクスにより腸内微生物相やその多様性を明らかにし,さらに真無盲腸類の種間,季節,標高による違いを解明した.研究では,真無盲腸類と同所的に分布するが生態学的・系統学的に異なるネズミ類との比較も行った.同位体分析は乾燥させた体毛を用い,種間,季節,標高を考慮して,C13とN15の同位体比をもとに食物連鎖において,同所的に分布する真無盲腸類がその生態学的位置を時系列で変化させていることを明らかにした.また,無脊椎動物への食物としての依存についても検証し,メタバーコーディングにより得られた結果との比較を進めている.一連の研究で得られた成果について現在投稿論文としての作成を進めている.

Research Progress Status

平成30年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

平成30年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (25 results)

All 2019 2018 2017 2016

All Journal Article (20 results) (of which Int'l Joint Research: 20 results,  Peer Reviewed: 20 results,  Open Access: 9 results,  Acknowledgement Compliant: 3 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Systematics and macroevolution of extant and fossil scalopine moles (Mammalia, Talpidae)2019

    • Author(s)
      Schwermann Achim H.、He Kai、Peters Benjamin J.、Plogschties Thorsten、Sansalone Gabriele
    • Journal Title

      Palaeontology

      Volume: - Issue: 4 Pages: 661-676

    • DOI

      10.1111/pala.12422

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A new genus of Asiatic short-tailed shrew (Soricidae, Eulipotyphla) based on molecular and morphological comparisons2018

    • Author(s)
      Kai He, Xing Chen, Peng Chen, Shui-Wang He, Feng Cheng, Xue-Long Jiang, Kevin Campbell
    • Journal Title

      Zoological Research

      Volume: 39 Issue: 5 Pages: 321-334

    • DOI

      10.24272/j.issn.2095-8137.2018.058

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Taxonomic revision of the genus Mesechinus (Mammalia: Erinaceidae) with description of a new species2018

    • Author(s)
      Huai-Sen Ai*, Kai He*,#, Zhong-Zheng Chen*, Jia-Qi Li, Tao Wan, Quan Li, Wen-Hui Nie, Jin-Huan Wang, Wei-Ting Su, Xue-Long Jiang
    • Journal Title

      Zoological Research

      Volume: 39 Issue: 5 Pages: 335-347

    • DOI

      10.24272/j.issn.2095-8137.2018.034

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Species identification of crested gibbons (Nomascus) in captivity in China using karyotyping- and PCR-based approaches2018

    • Author(s)
      Wenhui Nie#, Jinhuan Wang, Wei-Ting Su, Yi Hu, Shuiwang He, Xuelong Jiang#, Kai He
    • Journal Title

      Zoological Research

      Volume: 39 Issue: 5 Pages: 356-363

    • DOI

      10.24272/j.issn.2095-8137.2018.036

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] How many species of Apodemus and Rattus occur in China? A survey based on mitochondrial cyt b and morphological analyses2018

    • Author(s)
      Shao-Ying Liu, Kai He, Shun-De Chen, Wei Jin, Robert W. Murphy, Ming-Kun Tang, Rui Liao, Feng-Jun Li
    • Journal Title

      Zoological Research

      Volume: 39 Issue: 5 Pages: 309-320

    • DOI

      10.24272/j.issn.2095-8137.2018.053

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fossil gibbons (Mammalia, Hylobatidae) from the Pleistocene of Chongzuo, Guangxi Zhuang Autonomous2018

    • Author(s)
      Ying-Qi Zhang, Chang-Zhu Jin, Yuan Wang, Alejandra Ortiz, Kai He, Terry Harrison
    • Journal Title

      Vertebrata PalAsiatica

      Volume: 56 Pages: 248-263

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dispersal and female philopatry in a long-term, stable, polygynous gibbon population: Evidence from 16 years field observation and genetics2018

    • Author(s)
      Hu Naiqing、Guan Zhenhua、Huang Bei、Ning Wenhe、He Kai、Fan Pengfei、Jiang Xuelong
    • Journal Title

      American Journal of Primatology

      Volume: 80 Issue: 9

    • DOI

      10.1002/ajp.22922

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Climate niche conservatism and complex topography illuminate the cryptic diversification of Asian shrew-like moles2018

    • Author(s)
      Wan Tao、He Kai、Jin Wei、Liu Shao-Ying、Chen Zhong-Zheng、Zhang Bin、Murphy Robert W.、Jiang Xue-Long
    • Journal Title

      Journal of Biogeography

      Volume: 45 Issue: 10 Pages: 2400-2414

    • DOI

      10.1111/jbi.13401

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular phylogeny and taxonomy of subgenus Eothenomys (Cricetidae: Arvicolinae: Eothenomys) with the description of four new species from Sichuan, China2018

    • Author(s)
      Liu Shao-Ying、Chen Shun-De、He Kai、Tang Mingkun、Liu Yang、Jin Wei、Li Song、Li Quan、Zeng Tao、Sun Zhi-Yu、Fu Jian-Rong、Liao Rui、Meng Yang、Wang Xin、Jiang Xue-Long、Murphy Robert W
    • Journal Title

      Zoological Journal of the Linnean Society

      Volume: - Issue: 2 Pages: 569-598

    • DOI

      10.1093/zoolinnean/zly071

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Taxonomy based on science is necessary for global conservation2018

    • Author(s)
      Thomson, S.A.ほか182名(71/183)
    • Journal Title

      PLoS Biology

      Volume: 16 Issue: 3 Pages: e2005075-e2005075

    • DOI

      10.1371/journal.pbio.2005075

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mitochondrial DNA analyses and ecological niche modeling reveal post-LGM expansion of the Assam macaque (Macaca assamensis) in the foothills of Nepal Himalaya2018

    • Author(s)
      Khanal Laxman、Chalise Mukesh K.、He Kai、Acharya Bipin K.、Kawamoto Yoshi、Jiang Xuelong
    • Journal Title

      American Journal of Primatology

      Volume: 80 Issue: 3

    • DOI

      10.1002/ajp.22748

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Altered hemoglobin co-factor sensitivity does not underlie the evolution of derived fossorial specializations in the family Talpidae2018

    • Author(s)
      Campbell, K.L., Gaudry, M.J., He, K., Suzuki, H., Zhang, Y.-p., Jiang, X.-L., Weber, R.E.
    • Journal Title

      Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology

      Volume: - Pages: 150-155

    • DOI

      10.1016/j.cbpb.2018.01.001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] First records of Indochinese Arboreal Niviventer (Chiromyscus langbianis) in Yunnan Province, China2018

    • Author(s)
      Feng Cheng, Zhongzheng Chen, Bin Zhang, Kai He, Xuelong Jiang
    • Journal Title

      Acta Theriologica Sinica

      Volume: 38 Pages: 217-220

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Capture Hybridization of Long-Range DNA Fragments for High-Throughput Sequencing2018

    • Author(s)
      Chen, X., Ni, G., He, K., Ding, Z.-l., Li, G.-m., Adeola, A.C., Murphy, R.W., Wang, W.-z., Zhang, Y.-P.
    • Journal Title

      Huang T, editor. Computational Systems Biology: Methods and Protocols. Springer New York.

      Volume: - Pages: 29-44

    • DOI

      10.1007/978-1-4939-7717-8_3

    • ISBN
      9781493977161, 9781493977178
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] First records of Taiwanese Gray Shrew (Crocidura tanakae) in Yunnan Province, China2017

    • Author(s)
      CHENG Feng, WAN Tao, CHEN Zhong-Zheng, Narayan Prasad Koju, HE Kai, JIANG Xue-Long
    • Journal Title

      Chinese Journal of Zoology

      Volume: 52 Pages: 865-869

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phylogeny and systematic revision of the genus Typhlomys (Rodentia, Platacanthomyidae): with description of new species.2017

    • Author(s)
      Feng Cheng,Kai He, Zhong-Zheng Chen, Bin Zhang, Tao Wan, Bao-Wei Zhang, Xue-Long Jiang.
    • Journal Title

      Journal of Mammalogy

      Volume: - Issue: 3 Pages: 731-743

    • DOI

      10.1093/jmammal/gyx016

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Description of a new species of Hoolock gibbon (Primates: Hylobatidae) based on integrative taxonomy2017

    • Author(s)
      Fan, Peng-Fei, He, Kai, Chen, Xing, Ortiz, Alejandra, Zhang, Bin, Zhao, Chao, Li, Yun-Qiao, Zhang, Hai-Bo, Kimock, Clare, Wang, Wen-Zhi, Groves, Colin, Turvey, Samuel T., Roos, Christian, Helgen, Kristofer M. and Jiang, Xue-Long
    • Journal Title

      American Journal of Primatology

      Volume: 79 Issue: 5

    • DOI

      10.1002/ajp.22631

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Talpid mole phylogeny unites shrew moles and illuminates overlooked cryptic species diversity2017

    • Author(s)
      He, Kai, Shinohara, Akio, Helgen, Kristofer M., Springer, Mark S., Jiang, Xue-Long and Campbell, Kevin L.
    • Journal Title

      Molecular Biology and Evolution

      Volume: 34 Issue: 1 Pages: 78-87

    • DOI

      10.1093/molbev/msw221

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Multilocus approaches reveal underestimated species diversity and inter-specific gene flow in pikas (Ochotona) from southwestern China2017

    • Author(s)
      Koju, Narayan Prasad, He, Kai, Chalise, Mukesh Kumar, Ray, Chris, Chen, Zhongzheng, Zhang, Bin, Wan, Tao, Chen, Shunde and Jiang, Xuelong.
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: 107 Pages: 237-245

    • DOI

      10.1016/j.ympev.2016.11.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Multi-locus phylogeny using topotype specimens sheds light on the systematics of Niviventer (Rodentia, Muridae) in China.2016

    • Author(s)
      Zhang, Bin, He, Kai, Wan, Tao, Chen, Peng, Sun, Guozheng, Liu, Shaoying, Nguyen, Truong Son, Lin, Liangkong and Jiang, Xuelong
    • Journal Title

      BMC Evolutionary Biology

      Volume: 16 Issue: 1

    • DOI

      10.1186/s12862-016-0832-8

    • NAID

      120006338060

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Niche partitioning and Gut microbiome of small mammals shaped by environmental habitats and seasonal change2018

    • Author(s)
      He Kai, 斉藤浩明, 本川雅治
    • Organizer
      The 2018 Annual Meeting of the Mammal Society of Japan
    • Related Report
      2018 Annual Research Report
  • [Presentation] Uncover small mammal diversity using integrative approaches with implication on the mammal fauna in northern Myanmar2017

    • Author(s)
      Kai HE, Jin-Wen SHI, Masaharu MOTOKAWA
    • Organizer
      Kyoto University and University of Yangon International Symposium/ AVIS2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Seasonality of small mammal communities in Mt. Nishikoma (Nagano), and its potential toward the understanding of ecological network (preliminary data)2017

    • Author(s)
      Kai HE, 斉藤浩明, 本川雅治
    • Organizer
      The 2017 Annual Meeting of the Mammal Society of Japan
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cryptic diversity in an enigmatic mammal species from the sky islands of southwestern China2016

    • Author(s)
      Kai He, Tao Wan, Klaus-Peter Koepfli, Xue-Long Jiang
    • Organizer
      The 22nd International Congress of Zoology
    • Place of Presentation
      沖縄科学技術大学院大学(沖縄県・恩納村)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A new taxon of hoolock gibbon from Southwest China2016

    • Author(s)
      Fan, Peng-Fei, He, Kai, Chen, Xing, Ortiz, Alejandra, Zhang, Bin, Zhao, Chao, Li, Yun-Qiao, Zhang, Hai-Bo, Kimock, Clare, Wang, Wen-Zhi, Groves, Colin, Turvey, Samuel T., Roos, Christian, Helgen, Kristofer M. and Jiang, Xue-Long
    • Organizer
      日本哺乳類学会2016年大会
    • Place of Presentation
      つくば大学(茨城県・つくば市)
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-10-11   Modified: 2024-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi