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Elucidation of the evolutionary mechanism behind the origin and diversification of insect wings and its application

Research Project

Project/Area Number 16H02596
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Niimi Teruyuki  基礎生物学研究所, 進化発生研究部門, 教授 (00293712)

Co-Investigator(Kenkyū-buntansha) 大出 高弘  基礎生物学研究所, 進化発生研究部門, 助教 (60742111)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2018: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Keywords翅 / 起源 / 多様性
Outline of Final Research Achievements

This research aims to understand the molecular mechanisms underlying insect wing diversity. This was done by utilizing a new approach of introducing the latest gene function analysis technology and next-generation sequencing technology, while using genes that are the key to wing evolution. We targeted its application based on the results of our research. As a result, we have succeeded in the draft genome sequencing, as well as in establishing CRISPR/Cas9-based heritable targeted mutagenesis in the firebrat, Thermobia domestica. In addition, we succeeded in producing a novel winged flightless ladybird beetle based on the RNAi method, which is considered to be more useful in the development of biopesticides.

Academic Significance and Societal Importance of the Research Achievements

本研究によって得られる成果から、昆虫の独自性の理解に留まらず、広い生物種で認められる共通したボディプランの分子機構からいかに多様な形態が形成されるかという一般生物学に還元できる根本的な問題について多くの理解が得られることが期待される。
本研究では、基礎的な研究にとどまらず、本研究から得られた知見を活用して、ゲノム編集やRNAi法を利用した新規天敵昆虫の開発に資する。この技術は、従来にはない新規の生物農薬の開発に有用な基盤となることにとどまらず、様々な人為的な改変有用昆虫への応用が期待される。

Report

(3 results)
  • 2019 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results)

  • [Journal Article] Molecular characterization of eye pigmentation-related ABC transporter genes in the ladybird beetle Harmonia axyridis reveals striking gene duplication of the white gene.2018

    • Author(s)
      Tsuji, T., Gotoh, H., Morita, S., Hirata, J., Minakuchi, Y., Yaginuma, T., Toyoda, A. and Niimi, T.
    • Journal Title

      Zool. Sci.

      Volume: 印刷中

    • NAID

      210000170345

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A method for cryopreservation of ovaries of the ladybird beetle, Harmonia axyridis.2018

    • Author(s)
      Kawaguchi, H. and Niimi, T.
    • Journal Title

      J. Insect Biotechnol. Sericol.

      Volume: 87

    • NAID

      130007431353

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] ナミテントウの卵巣凍結保存2018

    • Author(s)
      川口はるか・新美輝幸
    • Organizer
      平成30年度 蚕糸・昆虫機能利用学術講演会 -日本蚕糸学会第88回大会-
    • Related Report
      2017 Annual Research Report
  • [Presentation] 翅形成遺伝子群は昆虫の背板縁形成に機能する2018

    • Author(s)
      大出高弘・三戸太郎・新美輝幸
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] On the evolutionary origin of insect wings: comparison of development between basal pterygote and apterygote insects.2017

    • Author(s)
      Ohde, T. and Niimi T.
    • Organizer
      第10回Evo-Devo青年の会
    • Related Report
      2017 Annual Research Report
  • [Presentation] What is the key developmental change for insect wing evolution?2017

    • Author(s)
      Ohde, T., Mito, T. and Niimi, T.
    • Organizer
      2nd Biennial Meeting of Pan-American Society for Evolutionary Developmental Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] はじめに2017

    • Author(s)
      新美輝幸
    • Organizer
      日本進化学会第19回大会 シンポジウム “エボデボから見る非モデル生物研究の魅力”
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] できるかな?ナミテントウの卵巣凍結保存2017

    • Author(s)
      川口はるか・新美輝幸
    • Organizer
      昆虫ポストゲノム研究会 2017年 in つくば
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナミテントウの卵巣凍結保存の試み2017

    • Author(s)
      川口はるか・新美輝幸
    • Organizer
      Cryopreservation Conference 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] The role of wing formation genes in non-wing developmental contexts2016

    • Author(s)
      Takahiro Ohde and Teruyuki Niimi
    • Organizer
      The joint meeting of the 22nd International Congress of Zoology and the Meeting of the Zoological Society of Japan
    • Place of Presentation
      Okinawa Convention Center, Ginowan, Okinawa, Japan
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evo-devo study of insect wing and its serial homologs: from apterygotes to pterygotes2016

    • Author(s)
      Takahiro Ohde and Teruyuki Niimi
    • Organizer
      XXV International Congress of Entomology 2016
    • Place of Presentation
      Orange County Convention Center, Orlando, FL, USA
    • Year and Date
      2016-09-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 昆虫形態進化研究におけるボトムアップデザインアプローチ2016

    • Author(s)
      大出高弘・新美輝幸
    • Organizer
      昆虫デザイン研究会
    • Place of Presentation
      岡崎コンファレンスセンター,愛知県岡崎市
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 昆虫の翅は作れるのか?2016

    • Author(s)
      大出高弘
    • Organizer
      第9回Evo-Devo青年の会
    • Place of Presentation
      岡崎コンファレンスセンター,愛知県岡崎市
    • Year and Date
      2016-06-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 翅と翅連続相同物間における遺伝子発現の保存性について2016

    • Author(s)
      大出高弘・新美輝幸
    • Organizer
      第52回日本節足動物発生学会
    • Place of Presentation
      湘南国際村センター,神奈川県横須賀市
    • Year and Date
      2016-06-11
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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